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An innovative multi-enzymatic system for gluconic acid production from starch using Aspergillus niger whole-cells.
Pucci, Emanoela Fernanda Queiroz; Buffo, Mariane Molina; Del Bianco Sousa, Marina; Tardioli, Paulo Waldir; Badino, Alberto Colli.
Afiliação
  • Pucci EFQ; Graduate Program in Chemical Engineering, Federal University of São Carlos, São Carlos, SP, Brazil.
  • Buffo MM; Laboratory of Fermentation Processes, Department of Chemical Engineering, Federal University of São Carlos, São Carlos, SP, Brazil.
  • Del Bianco Sousa M; Laboratory of Fermentation Processes, Department of Chemical Engineering, Federal University of São Carlos, São Carlos, SP, Brazil.
  • Tardioli PW; Graduate Program in Chemical Engineering, Federal University of São Carlos, São Carlos, SP, Brazil; Laboratory of Enzymatic Processes, Department of Chemical Engineering, Federal University of São Carlos, São Carlos, SP, Brazil. Electronic address: pwtardioli@ufscar.br.
  • Badino AC; Graduate Program in Chemical Engineering, Federal University of São Carlos, São Carlos, SP, Brazil; Laboratory of Fermentation Processes, Department of Chemical Engineering, Federal University of São Carlos, São Carlos, SP, Brazil. Electronic address: badinojr@ufscar.br.
Enzyme Microb Technol ; 171: 110309, 2023 Dec.
Article em En | MEDLINE | ID: mdl-37690395
The use of multi-enzymatic systems for the industrial production of chemical compounds is currently considered an important green tool in synthetic organic chemistry. Gluconic acid is a multi-functional organic acid widely used in the chemical, pharmaceutical, food, textile, and construction industries. Its industrial production from glucose by fermentation using Aspergillus niger has drawbacks including high costs related to cell growth and maintenance of cell viability. This study presents an innovative one-step multi-enzymatic system for gluconic acid production from starch using Aspergillus niger whole-cells in association with amylolytic enzymes. Using soluble starch as substrate, the following results were achieved for 96 h of reaction: 134.5 ± 4.3 g/L gluconic acid concentration, 98.2 ± 1.3 % gluconic acid yield, and 44.8 ± 1.4 gGA/gwhole-cells biocatalyst yield. Although the process has been developed using starch as raw material, the approach is feasible for any substrate or residue that can be hydrolyzed to glucose.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Enzyme Microb Technol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Enzyme Microb Technol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos