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Ancient Clostridium DNA and variants of tetanus neurotoxins associated with human archaeological remains.
Hodgins, Harold P; Chen, Pengsheng; Lobb, Briallen; Wei, Xin; Tremblay, Benjamin J M; Mansfield, Michael J; Lee, Victoria C Y; Lee, Pyung-Gang; Coffin, Jeffrey; Duggan, Ana T; Dolphin, Alexis E; Renaud, Gabriel; Dong, Min; Doxey, Andrew C.
Afiliação
  • Hodgins HP; Department of Biology and the Waterloo Centre for Microbial Research, University of Waterloo, Waterloo, ON, Canada.
  • Chen P; Department of Urology, Boston Children's Hospital, Boston, MA, USA.
  • Lobb B; Department of Surgery and Department of Microbiology, Harvard Medical School, Boston, MA, USA.
  • Wei X; Department of Biology and the Waterloo Centre for Microbial Research, University of Waterloo, Waterloo, ON, Canada.
  • Tremblay BJM; Department of Biology and the Waterloo Centre for Microbial Research, University of Waterloo, Waterloo, ON, Canada.
  • Mansfield MJ; Department of Biology and the Waterloo Centre for Microbial Research, University of Waterloo, Waterloo, ON, Canada.
  • Lee VCY; Genomics and Regulatory Systems Unit, Okinawa Institute of Science and Technology Graduate University, Onna, Okinawa, Japan.
  • Lee PG; Department of Biology and the Waterloo Centre for Microbial Research, University of Waterloo, Waterloo, ON, Canada.
  • Coffin J; Department of Urology, Boston Children's Hospital, Boston, MA, USA.
  • Duggan AT; Department of Surgery and Department of Microbiology, Harvard Medical School, Boston, MA, USA.
  • Dolphin AE; Department of Anthropology, University of Waterloo, Waterloo, ON, Canada.
  • Renaud G; McMaster Ancient DNA Centre, Department of Anthropology, McMaster University, Hamilton, ON, Canada.
  • Dong M; Department of Anthropology, University of Waterloo, Waterloo, ON, Canada.
  • Doxey AC; Department of Health Technology, Section of Bioinformatics, Technical University of Denmark, Kongens Lyngby, Denmark. gabre@dtu.dk.
Nat Commun ; 14(1): 5475, 2023 09 06.
Article em En | MEDLINE | ID: mdl-37673908
The analysis of microbial genomes from human archaeological samples offers a historic snapshot of ancient pathogens and provides insights into the origins of modern infectious diseases. Here, we analyze metagenomic datasets from 38 human archaeological samples and identify bacterial genomic sequences related to modern-day Clostridium tetani, which produces the tetanus neurotoxin (TeNT) and causes the disease tetanus. These genomic assemblies had varying levels of completeness, and a subset of them displayed hallmarks of ancient DNA damage. Phylogenetic analyses revealed known C. tetani clades as well as potentially new Clostridium lineages closely related to C. tetani. The genomic assemblies encode 13 TeNT variants with unique substitution profiles, including a subgroup of TeNT variants found exclusively in ancient samples from South America. We experimentally tested a TeNT variant selected from an ancient Chilean mummy sample and found that it induced tetanus muscle paralysis in mice, with potency comparable to modern TeNT. Thus, our ancient DNA analysis identifies DNA from neurotoxigenic C. tetani in archaeological human samples, and a novel variant of TeNT that can cause disease in mammals.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tétano / DNA Antigo Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals / Humans País/Região como assunto: America do sul / Chile Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Canadá País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tétano / DNA Antigo Tipo de estudo: Prognostic_studies / Risk_factors_studies Limite: Animals / Humans País/Região como assunto: America do sul / Chile Idioma: En Revista: Nat Commun Assunto da revista: BIOLOGIA / CIENCIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Canadá País de publicação: Reino Unido