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Selection of Biodegrading Phytosterol Strains.
Mondaca, María-Angélica; Vidal, Maricel; Chamorro, Soledad; Vidal, Gladys.
Afiliação
  • Mondaca MA; Microbiology Department, University of Concepción, Concepción, Chile.
  • Vidal M; Microbiology Department, University of Concepción, Concepción, Chile.
  • Chamorro S; Water Research Center for Agriculture and Mining (CRHIAM), ANID Fondap Center, Victoria, Concepción, Chile.
  • Vidal G; Engineering and Environmental Biotechnology Group, Environmental Science Faculty & Center EULA-Chile, University of Concepción, Concepción, Chile.
Methods Mol Biol ; 2704: 43-50, 2023.
Article em En | MEDLINE | ID: mdl-37642837
The phytosterol-biotransforming strains can be selected from Mycobacterium sp. using a high concentration of ß-sitosterol. The selection is made by culturing the strains in a medium enriched with 14 g/L of ß-sitosterol as the unique source of carbon. During 2 months, the bacterial cultures are transferred successively. The extraction of the biotransformation products is made with methanol and ethyl acetate. The qualitative and quantitative analyses are made by means of thin-layer chromatography, gas-liquid chromatography (GLC), and GLC-mass spectrometry. Under these conditions, it is observed that after seven transfers, the strains Mycobacterium sp. MB-3683 and Mycobacterium fortuitum B-11045 increase their biotransformation capacity from 20% to 64% and from 34% to 55%, respectively. The products in the highest proportion identified for each trial are androstenedione and androstadienedione. The results suggest that the high substrate concentration could be a selective mechanism to obtain strains more efficient in the biotransformation of ß-sitosterol into steroidal bases.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fitosteróis Tipo de estudo: Qualitative_research Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fitosteróis Tipo de estudo: Qualitative_research Idioma: En Revista: Methods Mol Biol Assunto da revista: BIOLOGIA MOLECULAR Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos