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Comparison of Four Purification Methods on Serum Extracellular Vesicle Recovery, Size Distribution, and Proteomics.
Jimenez, Dianny Elizabeth; Tahir, Muhammad; Faheem, Muhammad; Alves, Wellington Bruno Dos Santos; Correa, Barbara de Lucena; Andrade, Gabriel Rocha de; Larsen, Martin R; Oliveira, Getulio Pereira de; Pereira, Rinaldo Wellerson.
Afiliação
  • Jimenez DE; Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília 71966-700, Brazil.
  • Tahir M; Department of Biochemistry & Molecular Biology, University of Southern Denmark, 5230 Odense, Denmark.
  • Faheem M; Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília 71966-700, Brazil.
  • Alves WBDS; Department of Biomedical Sciences, University of North Dakota School of Medicine & Health Sciences, Grand Forks, ND 58202, USA.
  • Correa BL; Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília 71966-700, Brazil.
  • Andrade GR; Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília 71966-700, Brazil.
  • Larsen MR; Programa de Pós-Graduação em Ciências Genômicas e Biotecnologia, Universidade Católica de Brasília, Brasília 71966-700, Brazil.
  • Oliveira GP; Department of Biochemistry & Molecular Biology, University of Southern Denmark, 5230 Odense, Denmark.
  • Pereira RW; The Barnett Institute for Chemical & Biological Analysis, Northeastern University, Boston, MA 02115, USA.
Proteomes ; 11(3)2023 Jul 25.
Article em En | MEDLINE | ID: mdl-37606419
In recent decades, the role played by extracellular vesicles in physiological and pathological processes has attracted attention. Extracellular vesicles are released by different types of cells and carry molecules that could become biomarkers for the diagnosis of diseases. Extracellular vesicles are also moldable tools for the controlled release of bioactive substances in clinical and therapeutic applications. However, one of the significant challenges when studying these exciting and versatile vesicles is the purification process, which presents significant difficulties in terms of lack of purity, yield, and reproducibility, reflected in unreliable data. Therefore, our objective in the present study was to compare the proteomic profile of serum-derived EVs purified using ExoQuick™ (Systems Biosciences), Total Isolation Kit (Life Technologies), Ultracentrifugation, and Ultrafiltration. Each technique utilized for purification has shown different concentrations and populations of purified particles. The results showed marked differences in distribution, size, and protein content, demonstrating the need to develop reproducible and reliable protocols to isolate extracellular vesicles for their clinical application.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Guideline Idioma: En Revista: Proteomes Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Guideline Idioma: En Revista: Proteomes Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça