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Morphometric assessment of cryopreserved livestock bull spermatozoa in the tropics.
Víquez, Luis; Sevilla, Francisco; Araya-Zúñiga, Ignacio; Soler, Carles; Barquero, Vinicio; Roldan, Eduardo R S; Valverde, Anthony.
Afiliação
  • Víquez L; School of Agronomy, Animal Reproduction Laboratory, Costa Rica Institute of Technology, Alajuela, San Carlos, Costa Rica.
  • Sevilla F; School of Agronomy, Animal Reproduction Laboratory, Costa Rica Institute of Technology, Alajuela, San Carlos, Costa Rica.
  • Araya-Zúñiga I; School of Agronomy, Animal Reproduction Laboratory, Costa Rica Institute of Technology, Alajuela, San Carlos, Costa Rica.
  • Soler C; Department of Cellular Biology, Functional Biology and Physical Anthropology, Faculty of Biological Sciences, University of Valencia, Burjassot, Spain.
  • Barquero V; Faculty of Agri-Food Sciences, Alfredo Volio Mata Experimental Station, University of Costa Rica, Cartago, Costa Rica.
  • Roldan ERS; Department of Biodiversity and Evolutionary Biology, National Museum of Natural Sciences, Spanish National Research Council (CSIC), Madrid, Spain.
  • Valverde A; School of Agronomy, Animal Reproduction Laboratory, Costa Rica Institute of Technology, Alajuela, San Carlos, Costa Rica.
Reprod Domest Anim ; 58(10): 1439-1447, 2023 Oct.
Article em En | MEDLINE | ID: mdl-37603017
The identification of different morphometric patterns of spermatozoa serves as a basis for improving our understanding of the diversity in an ejaculate and to relate them to the potential fertility of males. In this study, we aimed to examine the semen subpopulation structure, following dilution in semen of extenders, using a mathematical approach a possible application to fertility analyses. Ten sexually mature Bos taurus bulls were randomly allotted to one of three groups: (1) Tris-citric acid-egg yolk extender (Tris-EY); (2) commercial egg yolk extender OptiXcell® and (3) commercial egg yolk extender Triladyl®. The results showed significant differences (p < .05) between extenders in terms of values for head size and head shape variables of individual sperm, indicating an influence of extender composition. Sperm head width was found to significantly differ (p < .05) according to the extender, decreasing in the following order: OptiXcell® (4.836 ± 0.017 µm), Triladyl® (4.695 ± 0.012 µm) and Tris-EY (4.638 ± 0.010 µm). Principal component analysis allowed us to identify two subpopulations in OptiXcell®, and three subpopulations were each found in Triladyl® and Tris-EY. Overall, we observed significant differences between sperm subpopulations within each extender (p < .05), with differences in sperm head size and shape between bovine species that can be related to functionality and fertility capabilities.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: Reprod Domest Anim Assunto da revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Costa Rica País de publicação: Alemanha

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Prognostic_studies Idioma: En Revista: Reprod Domest Anim Assunto da revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Costa Rica País de publicação: Alemanha