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Rectal Cancer Tissue Lipidome Differs According to Response to Neoadjuvant Therapy.
Sánchez-Vinces, Salvador; Duarte, Gustavo Henrique Bueno; Messias, Marcia Cristina Fernandes; Gatinoni, Caroline Fernanda Alves; Silva, Alex Ap Rosini; Sanches, Pedro Henrique Godoy; Martinez, Carlos Augusto Real; Porcari, Andreia M; Carvalho, Patricia de Oliveira.
Afiliação
  • Sánchez-Vinces S; Health Sciences Postgraduate Program, São Francisco University-USF, Bragança Paulista, São Paulo 12916-900, Brazil.
  • Duarte GHB; Health Sciences Postgraduate Program, São Francisco University-USF, Bragança Paulista, São Paulo 12916-900, Brazil.
  • Messias MCF; Health Sciences Postgraduate Program, São Francisco University-USF, Bragança Paulista, São Paulo 12916-900, Brazil.
  • Gatinoni CFA; Health Sciences Postgraduate Program, São Francisco University-USF, Bragança Paulista, São Paulo 12916-900, Brazil.
  • Silva AAR; MS4Life Laboratory of Mass Spectrometry, Health Sciences Postgraduate Program, São Francisco University-USF, Bragança Paulista, São Paulo 12916-900, Brazil.
  • Sanches PHG; MS4Life Laboratory of Mass Spectrometry, Health Sciences Postgraduate Program, São Francisco University-USF, Bragança Paulista, São Paulo 12916-900, Brazil.
  • Martinez CAR; Department of Colorectal Surgery, São Francisco University-USF, Bragança Paulista, São Paulo 12916-900, Brazil.
  • Porcari AM; MS4Life Laboratory of Mass Spectrometry, Health Sciences Postgraduate Program, São Francisco University-USF, Bragança Paulista, São Paulo 12916-900, Brazil.
  • Carvalho PO; Health Sciences Postgraduate Program, São Francisco University-USF, Bragança Paulista, São Paulo 12916-900, Brazil.
Int J Mol Sci ; 24(14)2023 Jul 14.
Article em En | MEDLINE | ID: mdl-37511236
Rectal cancer (RC) is a gastrointestinal cancer with a poor prognosis. While some studies have shown metabolic reprogramming to be linked to RC development, it is difficult to define biomolecules, like lipids, that help to understand cancer progression and response to therapy. The present study investigated the relative lipid abundance in tumoral tissue associated with neoadjuvant therapy response using untargeted liquid chromatography-mass spectrometry lipidomics. Locally advanced rectal cancer (LARC) patients (n = 13), clinically staged as T3-4 were biopsied before neoadjuvant chemoradiotherapy (nCRT). Tissue samples collected before nCRT (staging) and afterwards (restaging) were analyzed to discover lipidomic differences in RC cancerous tissue from Responders (n = 7) and Non-responders (n = 6) to nCRT. The limma method was used to test differences between groups and to select relevant feature lipids from tissue samples. Simple glycosphingolipids and differences in some residues of glycerophospholipids were more abundant in the Non-responder group before and after nCRT. Oxidized glycerophospholipids were more abundant in samples of Non-responders, especially those collected after nCRT. This work identified potential lipids in tissue samples that take part in, or may explain, nCRT failure. These results could potentially provide a lipid-based explanation for nCRT response and also help in understanding the molecular basis of RC and nCRT effects on the tissue matrix.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Retais / Terapia Neoadjuvante Limite: Humans Idioma: En Revista: Int J Mol Sci Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias Retais / Terapia Neoadjuvante Limite: Humans Idioma: En Revista: Int J Mol Sci Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça