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Ipconazole Induces Oxidative Stress, Cell Death, and Proinflammation in SH-SY5Y Cells.
Villaorduña, Carlos; Mendoza-Carlos, Mariano; Chuyma, Manuel; Avilés, Jhon; Avalos-Diaz, Ayda; Lozano-Reategui, Ronald; Garcia-Ruiz, Juan; Panduro-Tenazoa, Nadia; Vargas, Jessy; Moran-Quintanilla, Ysabel; Rodríguez, José-Luis.
Afiliação
  • Villaorduña C; Faculty of Pharmacy, Universidad Nacional Mayor de San Marcos, Lima 15021, Peru.
  • Mendoza-Carlos M; Agroforestry Department, Universidad Nacional Intercultural de la Amazonia, Pucallpa 25004, Peru.
  • Chuyma M; Agroforestry Department, Universidad Nacional Intercultural de la Amazonia, Pucallpa 25004, Peru.
  • Avilés J; Agroforestry Department, Universidad Nacional Intercultural de la Amazonia, Pucallpa 25004, Peru.
  • Avalos-Diaz A; Agroforestry Department, Universidad Nacional Intercultural de la Amazonia, Pucallpa 25004, Peru.
  • Lozano-Reategui R; Agroforestry Department, Universidad Nacional Intercultural de la Amazonia, Pucallpa 25004, Peru.
  • Garcia-Ruiz J; Agroforestry Department, Universidad Nacional Intercultural de la Amazonia, Pucallpa 25004, Peru.
  • Panduro-Tenazoa N; Agroforestry Department, Universidad Nacional Intercultural de la Amazonia, Pucallpa 25004, Peru.
  • Vargas J; Agroforestry Department, Universidad Nacional Intercultural de la Amazonia, Pucallpa 25004, Peru.
  • Moran-Quintanilla Y; Agroforestry Department, Universidad Nacional Intercultural de la Amazonia, Pucallpa 25004, Peru.
  • Rodríguez JL; Faculty of Veterinary, Universidad Complutense de Madrid, 28040 Madrid, Spain.
Toxics ; 11(7)2023 Jun 30.
Article em En | MEDLINE | ID: mdl-37505534
Ipconazole is an antifungal agrochemical widely used in agriculture against seed diseases of rice, vegetables, and other crops; due to its easy accumulation in the environment, it poses a hazard to human, animal, and environmental health. Therefore, we investigated the cytotoxic effect of ipconazole on SH-SY5Y neuroblastoma cells using cell viability tests (MTT), ROS production, caspase3/7 activity, and molecular assays of the biomarkers of cell death (Bax, Casp3, APAF1, BNIP3, and Bcl2); inflammasome (NLRP3, Casp1, and IL1ß); inflammation (NFκB, TNFα, and IL6); and antioxidants (NRF2, SOD, and GPx). SH-SY5Y cells were exposed to ipconazole (1, 5, 10, 20, 50, and 100 µM) for 24 h. The ipconazole, in a dose-dependent manner, reduced cell viability and produced an IC50 of 32.3 µM; it also produced an increase in ROS production and caspase3/7 enzyme activity in SH-SY5Y cells. In addition, ipconazole at 50 µM induced an overexpression of Bax, Casp3, APAF1, and BNIP3 (cell death genes); NLRP3, Casp1, and IL1B (inflammasome complex genes); and NFκB, TNFα, and IL6 (inflammation genes); it also reduced the expression of NRF2, SOD, and GPx (antioxidant genes). Our results show that ipconazole produces cytotoxic effects by reducing cell viability, generating oxidative stress, and inducing cell death in SH-SY5Y cells, so ipconazole exposure should be considered as a factor in the presentation of neurotoxicity or neurodegeneration.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Toxics Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Peru País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Toxics Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Peru País de publicação: Suíça