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An electrochemical microfluidic device for non-enzymatic cholesterol determination using a lab-made disposable electrode.
Watanabe, Emily Yamagutti; Gevaerd, Ava; Caetano, Fabio Roberto; Marcolino-Junior, Luiz Humberto; Bergamini, Márcio Fernando.
Afiliação
  • Watanabe EY; Laboratório de Sensores Eletroquímicos (LabSensE) - Departamento de Química, Universidade Federal do Paraná (UFPR), Curitiba, CEP 81531-980, PR, Brazil. luiz1berto@ufpr.br.
  • Gevaerd A; Laboratório de Sensores Eletroquímicos (LabSensE) - Departamento de Química, Universidade Federal do Paraná (UFPR), Curitiba, CEP 81531-980, PR, Brazil. luiz1berto@ufpr.br.
  • Caetano FR; Hilab, Rua José Altair Possebom, 800, Curitiba, CEP 81270-185, PR, Brazil.
  • Marcolino-Junior LH; Laboratório de Sensores Eletroquímicos (LabSensE) - Departamento de Química, Universidade Federal do Paraná (UFPR), Curitiba, CEP 81531-980, PR, Brazil. luiz1berto@ufpr.br.
  • Bergamini MF; Laboratório de Sensores Eletroquímicos (LabSensE) - Departamento de Química, Universidade Federal do Paraná (UFPR), Curitiba, CEP 81531-980, PR, Brazil. luiz1berto@ufpr.br.
Anal Methods ; 15(30): 3692-3699, 2023 08 03.
Article em En | MEDLINE | ID: mdl-37469272
Cholesterol is an important steroid and hormone precursor, and its levels in the blood are associated with risk factors for cardiovascular diseases. In this work, a non-enzymatic methodology for cholesterol determination in serum samples is described. First, a working electrode was constructed using homemade ink and a plastic substrate by a simple dunking process. Next, the dunked electrode (DWE) was modified with nickel ions (Ni-DWE) and combined with a low-cost microfluidic platform, resulting in a thread-based electroanalytical device (µTED). The arrangement of µTED consists of two coupled electrodes (one reference in the inlet reservoir and an auxiliary electrode against the outlet reservoir) and a mobile support for facile working electrode exchange. After optimization of construction parameters, the system was applied for non-enzymatic determination of cholesterol under alkaline conditions using the redox pair Ni(II)/Ni(III) as a mediator. Under the best analytical conditions, a calibration curve was constructed with a linear dynamic range (LDR) from 0.25 to 25.0 µmol L-1, and the calculated limits of detection (LOD) and quantification (LOQ) were 0.074 and 0.24 µmol L-1, respectively. No effects of possible interferents on electrochemical response were found in the presence of ascorbic acid, uric acid, dopamine, cysteine, and glucose, suggesting that the proposed device can be used for the determination of cholesterol without significant matrix effects of human plasma. Finally, cholesterol analysis was carried out using spiked plasma samples, and good recovery values were achieved.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Eletroquímicas / Glucose Tipo de estudo: Risk_factors_studies Limite: Humans Idioma: En Revista: Anal Methods Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Eletroquímicas / Glucose Tipo de estudo: Risk_factors_studies Limite: Humans Idioma: En Revista: Anal Methods Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido