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A posteriori parameters from paternity tests of a Mexican laboratory with the powerplex fusion system.
Elizabeth García-Aceves, Mayra; Gabriela Jasso-Razo, Diana; Xitlally Díaz-Navarro, Xochitl; Rangel-Villalobos, Héctor.
Afiliação
  • Elizabeth García-Aceves M; Instituto de Investigación en Genética Molecular, Universidad de Guadalajara (CUCiénega-UdeG), Av. Universidad 1115, Paso Blanco, Ocotlán, Jalisco CP 47810, Mexico.
  • Gabriela Jasso-Razo D; Instituto de Investigación en Genética Molecular, Universidad de Guadalajara (CUCiénega-UdeG), Av. Universidad 1115, Paso Blanco, Ocotlán, Jalisco CP 47810, Mexico.
  • Xitlally Díaz-Navarro X; Laboratorio DNA Profile, Delgadillo Araujo #305, Col. El Rosario, Ocotlán, Jalisco CP 47849, Mexico.
  • Rangel-Villalobos H; Instituto de Investigación en Genética Molecular, Universidad de Guadalajara (CUCiénega-UdeG), Av. Universidad 1115, Paso Blanco, Ocotlán, Jalisco CP 47810, Mexico. Electronic address: hrangel@cuci.udg.mx.
Leg Med (Tokyo) ; 64: 102296, 2023 Sep.
Article em En | MEDLINE | ID: mdl-37463547
Population studies regarding Human identification (HID) systems report a priori forensic parameters, but rarely they describe a posteriori parameters from concluded paternity tests. We analyzed data from the PowerPlex® Fusion System in 1503 paternity tests from a Mexican laboratory for five years (2016-2020). The motherless duo paternity tests (89.8%) were more frequent than the standard trio tests (10.2%). A notable increase in motherless tests was noted regarding our previous report (89.8% vs 77.3%), probably explained by the COVID-19 pandemic. The estimated exclusion frequency in Mexico ranged from 30.1 (trio) to 32.1% (duo). For paternity exclusions, we report the number of mismatches and the frequency at which each STR was involved. The PowerPlex® Fusion system showed more than five mismatches in 100% of the standard trio tests excluding paternity, and the majority of motherless-duo tests (98.1%). In positive paternity tests, PowerPlex® Fusion offered a higher combined paternity index (PI) (average 1.18 E + 10) regarding HID systems with 15 and 20 STRs, even without the inclusion of the Y-linked locus DYS391 to the kinship interpretation. Individual and global STR mutation rates were estimated from 17 paternal mutations (µ = 0.0017), the majority involving a single-step mutation (94.11%). Five independent null alleles were detected, most of them involving the Penta E locus (80%), which suggests caution to the users working with DNA databases or kinship analysis, to avoid false exclusions with Penta E. In brief, our results provide a better overview of a posteriori informativeness offered by the PowerPlex® Fusion system for paternity testing in Mexico.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Paternidade / COVID-19 Limite: Humans País/Região como assunto: Mexico Idioma: En Revista: Leg Med (Tokyo) Assunto da revista: JURISPRUDENCIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: México País de publicação: Irlanda
Texto completo
Adicionar na Minha BVS
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Paternidade / COVID-19 Limite: Humans País/Região como assunto: Mexico Idioma: En Revista: Leg Med (Tokyo) Assunto da revista: JURISPRUDENCIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: México País de publicação: Irlanda