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Protective Effects of Fuziline on Dobutamine-Induced Heart Damage in Mice.
Hacanli, Yasemin; Aydin, Mehmet Salih; Ersöz, Ezhar Korkmaz; Kankiliç, Nazim; Koyuncu, Ismail; Güldür, Muhammet Emin; Temiz, Ebru; Dikme, Resat; Egi, Kadir; Çakmak, Yusuf; Padak, Mahmut.
Afiliação
  • Hacanli Y; Department of Cardiovascular Surgery, Medical School of Harran University, Sanliurfa, Turkey.
  • Aydin MS; Department of Cardiovascular Surgery, Medical School of Harran University, Sanliurfa, Turkey.
  • Ersöz EK; Department of Cardiovascular Surgery, Medical School of Harran University, Sanliurfa, Turkey.
  • Kankiliç N; Department of Cardiovascular Surgery, Medical School of Harran University, Sanliurfa, Turkey.
  • Koyuncu I; Departmant of Medicinal Biochemistry, Medical School of Harran University, Sanliurfa, Turkey.
  • Güldür ME; Department of Pathology, Islamic Science and Technology University, Gaziantep, Turkey.
  • Temiz E; Medical Promotion and Marketing Program, Health Services Vocational School, Harran University, Sanliurfa, Turkey.
  • Dikme R; Perfusion Techniques Program, Health Services Vocational School, Harran University, Sanliurfa, Turkey.
  • Egi K; Departmant of Medicinal Biochemistry, Medical School of Harran University, Sanliurfa, Turkey.
  • Çakmak Y; Animal Experiment Application and Research Center (HDAM), Harran University, Sanliurfa, Turkey.
  • Padak M; Perfusion Techniques Program, Health Services Vocational School, Harran University, Sanliurfa, Turkey.
Braz J Cardiovasc Surg ; 38(4): e20220251, 2023 07 04.
Article em En | MEDLINE | ID: mdl-37402273
INTRODUCTION: Fuziline is one of the many antioxidants currently being tested to treat cardiac damage. In our study, histopathological and biochemical effects of fuziline were investigated in mice with dobutamine-induced heart damage in vitro. METHODS: Thirty-two adult male BALB/c mice, average weight of 18-20 g, were randomly divided into four groups - Group 1 (sham, n=8), Group 2 (control, dobutamine, n=8), Group 3 (treatment 1, dobutamine + fuziline, n=8), and Group 4 (treatment 2, fuziline, n=8). Biochemical parameters and total antioxidant status (TAS), total oxidant status (TOS), and oxidative stress index (OSI) values were measured. Interleukin 1 beta (IL-1ß), NLR family, pyrin domain containing protein 3 (NLRP3), 8-hydroxy-deoxyguanosine (8-OHDG), gasdermin D (GSDMD), and galectin 3 (GAL-3) levels were analyzed by enzyme-linked immunosorbent assay method, and histopathological examination of heart tissues was performed. RESULTS: When dobutamine + fuziline and fuziline groups were compared, troponin-I (P<0.05), NLRP3 (P<0.001), GSDMD (P<0.001), 8-OHDG (P<0.001), IL-1ß (P<0.001), and GAL-3 (P<0.05) were found to be statistically significant. TOS level was the highest in the dobutamine group (P<0.001) and TAS level was the highest in the fuziline group (P<0.001). OSI level was statistically significant between the groups (P<0.001). In histopathological examination, focal necrosis areas were smaller in the dobutamine + fuziline group than in the dobutamine group, and cardiac myocytes were better preserved. CONCLUSION: Fuziline markedly reduced cardiac damage and pyroptosis in mice with dobutamine-induced heart damage by lowering the levels of GSDMD, 8-OHDG, IL-1ß, and GAL-3. It also prevented necrosis of cardiac myocytes in histopathological evaluation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dobutamina / Traumatismos Cardíacos Limite: Animals Idioma: En Revista: Braz J Cardiovasc Surg Assunto da revista: ANGIOLOGIA / CARDIOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Turquia País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Dobutamina / Traumatismos Cardíacos Limite: Animals Idioma: En Revista: Braz J Cardiovasc Surg Assunto da revista: ANGIOLOGIA / CARDIOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Turquia País de publicação: Brasil