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Identifying Key Genes and Functionally Enriched Pathways in Osteoporotic Patients by Weighted Gene Co-Expression Network Analysis.
Fan, Ping; Feng, Xiuyuan; Hu, Nan; Pu, Dan; He, Lan.
Afiliação
  • Fan P; Department of Rheumatism and Immunology, The First Affiliated Hospital of Xi'an Jiaotong University, No. 277 West Yanta Street, Xi'an, 710061, China. fanping@xjtu.edu.cn.
  • Feng X; Department of Rheumatism and Immunology, The First Affiliated Hospital of Xi'an Jiaotong University, No. 277 West Yanta Street, Xi'an, 710061, China.
  • Hu N; Department of Rheumatism and Immunology, The First Affiliated Hospital of Xi'an Jiaotong University, No. 277 West Yanta Street, Xi'an, 710061, China.
  • Pu D; Department of Rheumatism and Immunology, The First Affiliated Hospital of Xi'an Jiaotong University, No. 277 West Yanta Street, Xi'an, 710061, China.
  • He L; Department of Rheumatism and Immunology, The First Affiliated Hospital of Xi'an Jiaotong University, No. 277 West Yanta Street, Xi'an, 710061, China.
Biochem Genet ; 62(1): 436-451, 2024 Feb.
Article em En | MEDLINE | ID: mdl-37358674
Osteoporosis is a systemic bone disease characterized by low bone mineral density and bone microstructure damage, resulting in increased bone fragility and fracture risk. The present study aimed to identify key genes and functionally enriched pathways in osteoporotic patients. Weighted Gene Co-expression Network Analysis (WGCNA) was applied to microarray datasets of blood samples of osteoporotic patients from the Sao Paulo Ageing & Health [SPAH] study (26 osteoporotic samples and 31 normal samples) to construct co-expression networks and identify hub gene. The results showed that HDGF, AP2M1, DNAJC6, TMEM183B, MFSD2B, IGKV1-5, IGKV1-8, IGKV3-7, IGKV3D-11, and IGKV1D-42 are genes which were associated with the disease status of osteoporosis. Differentially expressed genes are enriched in proteasomal protein catabolic process, ubiquitin ligase complex, and ubiquitin-like protein transferase activity. Functional enrichment analysis demonstrated that genes in the tan module were enriched in immune-related functions, indicating that the immune system plays a critical role in osteoporosis. Validation assay demonstrated that the HDGF, AP2M1, TMEM183B, and MFSD2B levels were decreased in osteoporosis samples compared with healthy controls, while the levels of IGKV1-5, IGKV1-8, and IGKV1D-42 were increased in osteoporosis samples compared with healthy controls. In conclusion, our data identified and validated the association of HDGF, AP2M1, TMEM183B, MFSD2B, IGKV1-5, IGKV1-8, and IGKV1D-42 with osteoporosis in elderly women. These results suggest that these transcripts have potential clinical significance and may help to explain the molecular mechanisms and biological functions of osteoporosis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoporose / Perfilação da Expressão Gênica Limite: Aged / Female / Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Biochem Genet Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Osteoporose / Perfilação da Expressão Gênica Limite: Aged / Female / Humans País/Região como assunto: America do sul / Brasil Idioma: En Revista: Biochem Genet Ano de publicação: 2024 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos