The protective role of Wnt3a in peroxynitrite-induced damage of cochlear hair cells in vitro.
Braz J Otorhinolaryngol
; 89(4): 101278, 2023.
Article
em En
| MEDLINE
| ID: mdl-37331234
OBJECTIVE: To investigate the effect of peroxynitrite on the cultured cochlear hair cells of C57BL/6 P3 mice in vitro as well as the role of Wnt3a, as an activator of the canonical Wnt signaling pathway, underlying the action of such an oxidative stress. METHODS: The in vitro primary cultured cochlear hair cells were subjected to l00⯵M peroxynitrite and l00⯵M peroxynitrite +25â¯ng/mL Wnt3a for 24â¯h, the cell survival and morphological changes were examined by immunofluorescence and transmission electron microscopy. RESULTS: The number of surviving hair cells was significantly reduced in the 100⯵M peroxynitrite group, while it was significantly higher in the Wnt3aâ¯+â¯peroxynitrite treated group compared with the peroxynitrite treated group. The transmission electron microscopy showed that exposure to peroxynitrite induced a dramatic decrease in the number of mitochondria and severely disrupted mitochondrial ultrastructure, while Wnt3a clearly diminished the disruption of mitochondrial structure and preserved a higher number of mitochondria. CONCLUSION: These results indicated that peroxynitrite could cause oxidative damage to the cochlear hair cells, and low concentrations of Wnt3a has a protective effect against oxidative damage. LEVEL OF EVIDENCE: Level 2.
Palavras-chave
Texto completo:
1
Coleções:
01-internacional
Base de dados:
MEDLINE
Assunto principal:
Ácido Peroxinitroso
/
Células Ciliadas Auditivas
Limite:
Animals
Idioma:
En
Revista:
Braz J Otorhinolaryngol
Assunto da revista:
OTORRINOLARINGOLOGIA
Ano de publicação:
2023
Tipo de documento:
Article
País de afiliação:
China
País de publicação:
Brasil