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The protective role of Wnt3a in peroxynitrite-induced damage of cochlear hair cells in vitro.
Cui, Fengyun; Cao, Zhimin; Zhang, Qianru; Cao, Zhixin.
Afiliação
  • Cui F; Shandong Provincial Hospital Affiliated to Shandong First Medical University, Department of Pathology, Shandong Province, China.
  • Cao Z; Gao Tang People's Hospital Affiliated to Jining Medical University, Emergency Department, Shandong Province, China.
  • Zhang Q; Shandong Provincial Hospital Affiliated to Shandong First Medical University, Department of Pathology, Shandong Province, China.
  • Cao Z; Shandong Provincial Hospital Affiliated to Shandong First Medical University, Department of Pathology, Shandong Province, China. Electronic address: caozhixin12@126.com.
Braz J Otorhinolaryngol ; 89(4): 101278, 2023.
Article em En | MEDLINE | ID: mdl-37331234
OBJECTIVE: To investigate the effect of peroxynitrite on the cultured cochlear hair cells of C57BL/6 P3 mice in vitro as well as the role of Wnt3a, as an activator of the canonical Wnt signaling pathway, underlying the action of such an oxidative stress. METHODS: The in vitro primary cultured cochlear hair cells were subjected to l00 µM peroxynitrite and l00 µM peroxynitrite +25 ng/mL Wnt3a for 24 h, the cell survival and morphological changes were examined by immunofluorescence and transmission electron microscopy. RESULTS: The number of surviving hair cells was significantly reduced in the 100 µM peroxynitrite group, while it was significantly higher in the Wnt3a + peroxynitrite treated group compared with the peroxynitrite treated group. The transmission electron microscopy showed that exposure to peroxynitrite induced a dramatic decrease in the number of mitochondria and severely disrupted mitochondrial ultrastructure, while Wnt3a clearly diminished the disruption of mitochondrial structure and preserved a higher number of mitochondria. CONCLUSION: These results indicated that peroxynitrite could cause oxidative damage to the cochlear hair cells, and low concentrations of Wnt3a has a protective effect against oxidative damage. LEVEL OF EVIDENCE: Level 2.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ácido Peroxinitroso / Células Ciliadas Auditivas Limite: Animals Idioma: En Revista: Braz J Otorhinolaryngol Assunto da revista: OTORRINOLARINGOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ácido Peroxinitroso / Células Ciliadas Auditivas Limite: Animals Idioma: En Revista: Braz J Otorhinolaryngol Assunto da revista: OTORRINOLARINGOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: China País de publicação: Brasil