Your browser doesn't support javascript.
loading
N-(levodopa) chitosan derivative based on click chemistry shows biological functionality in brain cells.
Ortega-Fimbres, Olimpia; Montiel-Herrera, Marcelino; García-Villa, Denisse; Pérez-Delgado, Jonathan; Monge-Sanchez, Diana; Fernández-Quiroz, Daniel; de la Re-Vega, Enrique; Molina-Domínguez, Claudia; Angulo-Molina, Aracely; Castillo-Martín Del Campo, Claudia; Argüelles-Monal, Waldo; Astiazaran-García, Humberto; Preciado-Saldaña, Alejandra; Domínguez-Ávila, Abraham; González-Aguilar, Gustavo.
Afiliação
  • Ortega-Fimbres O; Department of Medicine and Health Sciences, University of Sonora, Hermosillo, Sonora, Mexico.
  • Montiel-Herrera M; Department of Medicine and Health Sciences, University of Sonora, Hermosillo, Sonora, Mexico.
  • García-Villa D; Department of Medicine and Health Sciences, University of Sonora, Hermosillo, Sonora, Mexico.
  • Pérez-Delgado J; Department of Medicine and Health Sciences, University of Sonora, Hermosillo, Sonora, Mexico.
  • Monge-Sanchez D; Department of Medicine and Health Sciences, University of Sonora, Hermosillo, Sonora, Mexico.
  • Fernández-Quiroz D; Department of Chemical Engineering and Metallurgy, University of Sonora, Hermosillo, Sonora, Mexico.
  • de la Re-Vega E; Department of Scientific and Technological Research, University of Sonora, Hermosillo, Sonora, Mexico.
  • Molina-Domínguez C; Department of Scientific and Technological Research, University of Sonora, Hermosillo, Sonora, Mexico.
  • Angulo-Molina A; Department of Chemistry, University of Sonora, Hermosillo, Sonora, Mexico.
  • Castillo-Martín Del Campo C; CIACYT , Autonomous University of San Luis Potosi, San Luis Potosi, Mexico.
  • Argüelles-Monal W; Research Center for Food and Development A. C, Hermosillo, Sonora, Mexico.
  • Astiazaran-García H; Research Center for Food and Development A. C, Hermosillo, Sonora, Mexico.
  • Preciado-Saldaña A; Research Center for Food and Development A. C, Hermosillo, Sonora, Mexico.
  • Domínguez-Ávila A; Research Center for Food and Development A. C, Hermosillo, Sonora, Mexico.
  • González-Aguilar G; Research Center for Food and Development A. C, Hermosillo, Sonora, Mexico.
Drug Dev Ind Pharm ; 49(7): 439-447, 2023 Jul.
Article em En | MEDLINE | ID: mdl-37310383
OBJECTIVE: To develop N-(levodopa) chitosan derivatives through click chemistry to study their effect in brain cells.Significance: This study presents a proof-of-concept that macromolecules such as N-(Levodopa) chitosan derivatives traverse brain cell membranes and induce biomedical functionalities. METHODS: Through click chemistry, we developed N-(levodopa) chitosan derivatives. They were physically and chemically characterized by FT-IR, 1H-NMR, TGA and Dynamic Light Scattering analyses. Solution and nanoparticles of N-(levodopa) chitosan derivatives were tested in primary cell cultures from the postnatal rat olfactory bulb, substantia nigra and corpus callosum. Ca2+ imaging and UPLC experiments were used to investigate if the biomaterial modulated the brain cell physiology. RESULTS: N-(levodopa) chitosan derivatives induced intracellular Ca2+ responses in primary cell cultures of the rat brain. UPLC experiments indicated that levodopa attached to chitosan was converted into dopamine by brain cells. CONCLUSION: The present study shows that N-(levodopa) chitosan may be useful to develop new treatment strategies, which could serve as molecular reservoirs of biomedical drugs to treat degenerative disorders of the nervous system.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Levodopa / Quitosana Limite: Animals Idioma: En Revista: Drug Dev Ind Pharm Ano de publicação: 2023 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Levodopa / Quitosana Limite: Animals Idioma: En Revista: Drug Dev Ind Pharm Ano de publicação: 2023 Tipo de documento: Article País de afiliação: México País de publicação: Reino Unido