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Ex Vivo Evaluation of the Efficacy of Photodynamic Therapy in Eliminating Enterococcus faecalis from Dentinal Tubules by Confocal Laser Scanning Microscopy.
Godoy, Roberta de Castro Leitão; Bueno, Carlos Eduardo da Silveira; De Martin, Alexandre Sigrist; Pelegrine, Rina Andrea; Fontana, Carlos Eduardo; Duarte, Marco Antônio Hungaro; Vivan, Rodrigo Ricci; Nascimento, Wayne Martins; Limoeiro, Ana Grasiela da Silva; Rocha, Daniel Guimarães Pedro.
Afiliação
  • Godoy RCL; Faculdade São Leopoldo Mandic, Department of Endodontics, Instituto de Pesquisas São Leopoldo Mandic, Campinas, São Paulo, Brazil.
  • Bueno CEDS; Faculdade São Leopoldo Mandic, Department of Endodontics, Instituto de Pesquisas São Leopoldo Mandic, Campinas, São Paulo, Brazil.
  • De Martin AS; Faculdade São Leopoldo Mandic, Department of Endodontics, Instituto de Pesquisas São Leopoldo Mandic, Campinas, São Paulo, Brazil.
  • Pelegrine RA; Faculdade São Leopoldo Mandic, Department of Endodontics, Instituto de Pesquisas São Leopoldo Mandic, Campinas, São Paulo, Brazil.
  • Fontana CE; Pontifical Catholic University of Campinas, Department of Endodontics, Center of Life Sciences, Campinas, São Paulo, Brazil.
  • Duarte MAH; Faculdade de Odontologia de Bauru, University of São Paulo, Bauru, São Paulo, Brazil.
  • Vivan RR; Faculdade de Odontologia de Bauru, University of São Paulo, Bauru, São Paulo, Brazil.
  • Nascimento WM; Faculdade São Leopoldo Mandic, Department of Endodontics, Instituto de Pesquisas São Leopoldo Mandic, Campinas, São Paulo, Brazil.
  • Limoeiro AGDS; Faculdade São Leopoldo Mandic, Department of Endodontics, Instituto de Pesquisas São Leopoldo Mandic, Campinas, São Paulo, Brazil. Electronic address: grasielalimoeiro@gmail.com.
  • Rocha DGP; Pontifical Catholic University of Campinas, Department of Endodontics, Center of Life Sciences, Campinas, São Paulo, Brazil.
J Endod ; 49(7): 889-893, 2023 Jul.
Article em En | MEDLINE | ID: mdl-37225038
INTRODUCTION: The aim of the present study was to investigate ex vivo by confocal laser scanning microscopy the antibacterial effect of photodynamic therapy (PDT) on dentinal tubules in the apical 5 mm of human mandibular premolars contaminated with Enterococcus faecalis. METHODS: Thirty-four teeth were standardized to 20 mm and foraminal anatomic diameters using a #20 K-file (Dentsply Maillefer). Samples were contaminated for 21 days and divided into the following 3 experimental groups (n = 10): the PDT group (instrumented canals and PDT), the passive ultrasonic irrigation (PUI) group (instrumented canals and PUI), and the PUI-PDT group (instrumented canals, PUI, and PDT), along with a control group (n = 4) (noninstrumented canals). The canals in the experimental groups were instrumented with ProTaper Next (Dentsply Maillefer) up to X3 and rinsed with EDTA and sodium hypochlorite. The photosensitizer used was 0.01% methylene blue with a preirradiation time of 5 minutes and a diode laser with 4 J energy and a 660-nm wavelength. Cross sections were made 5 mm from the apex of all samples, which were analyzed using confocal laser scanning microscopy. The results were analyzed using the Shapiro-Wilk and Kruskal-Wallis (Dunn) tests. RESULTS: There was a lower percentage of live bacteria in the PUI-PDT group, with a statistical difference compared with the control and PDT groups (P < .05). There was no statistical difference in the percentage of live bacteria between PUI-PDT and PUI (P > .05). CONCLUSIONS: It was concluded that the PUI-PDT association was most effective in disinfecting root canals compared with the control group and PDT.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fotoquimioterapia / Enterococcus faecalis Limite: Humans Idioma: En Revista: J Endod Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fotoquimioterapia / Enterococcus faecalis Limite: Humans Idioma: En Revista: J Endod Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos