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Comparison of 2 sampling methods for molecular detection of bacteria or fungi from feline hair and scale specimens.
Santana, Aline E; Torres, Sheila M F; Costa, Matheus de O.
Afiliação
  • Santana AE; Department of Internal Medicine, School of Veterinary Medicine and Animal Science, University of São Paulo, São Paulo, SP, Brazil.
  • Torres SMF; Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, São Paulo, SP, Brazil.
  • Costa MO; Veterinary Clinical Sciences, College of Veterinary Medicine, University of Minnesota, Saint Paul, MN, USA.
J Vet Diagn Invest ; 35(4): 384-389, 2023 Jul.
Article em En | MEDLINE | ID: mdl-37203881
Skin diseases of cats are among the most frequent client motivations for a veterinary consultation. Both carpet and toothbrush sampling are commonly used to obtain hair and scale samples for microbiologic testing. Although molecular tests have become more accessible and more widely used by clinicians, the ideal collection method for clinical specimens is unclear. To assess their performance in retrieving microbial DNA from clinical samples, we compared the bacterial and fungal DNA load in hair and skin scale samples collected using carpet or toothbrush methods. We evaluated sample DNA yield using fluorometry, spectrophotometry, and quantitative PCR. Despite no measurable differences in sample weight, toothbrush samples yielded significantly higher bacterial (p = 0.028) and fungal (p = 0.005) DNA loads compared to carpet samples, regardless of disease status. The toothbrush method was more effective in harvesting microbial DNA from hair and skin scale samples.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bactérias / Cabelo Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: J Vet Diagn Invest Assunto da revista: MEDICINA VETERINARIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bactérias / Cabelo Tipo de estudo: Diagnostic_studies Limite: Animals Idioma: En Revista: J Vet Diagn Invest Assunto da revista: MEDICINA VETERINARIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos