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A novel soybean hairy root system for gene functional validation.
Pereira, Bruna Medeiros; Arraes, Fabrício; Martins, Andressa Cunha Quintana; Alves, Nayara Sabrina Freitas; Melo, Bruno Paes; Morgante, Carolina Vianna; Saraiva, Mario Alfredo Passos; Grossi-de-Sá, Maria Fátima; Guimaraes, Patricia Messenberg; Brasileiro, Ana Cristina Miranda.
Afiliação
  • Pereira BM; EMBRAPA Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil.
  • Arraes F; EMBRAPA Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil.
  • Martins ACQ; EMBRAPA Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil.
  • Alves NSF; EMBRAPA Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil.
  • Melo BP; EMBRAPA Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil.
  • Morgante CV; Instituto Nacional de Ciência e Tecnologia-INCT PlantStress Biotech-Embrapa, Brasília, DF, Brazil.
  • Saraiva MAP; EMBRAPA Semiárido, Petrolina, PE, Brazil.
  • Grossi-de-Sá MF; EMBRAPA Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil.
  • Guimaraes PM; Instituto Nacional de Ciência e Tecnologia-INCT PlantStress Biotech-Embrapa, Brasília, DF, Brazil.
  • Brasileiro ACM; EMBRAPA Recursos Genéticos e Biotecnologia, Brasília, DF, Brazil.
PLoS One ; 18(5): e0285504, 2023.
Article em En | MEDLINE | ID: mdl-37200365
Agrobacterium rhizogenes-mediated transformation has long been explored as a versatile and reliable method for gene function validation in many plant species, including soybean (Glycine max). Likewise, detached-leaf assays have been widely used for rapid and mass screening of soybean genotypes for disease resistance. The present study combines these two methods to establish an efficient and practical system to generate transgenic soybean hairy roots from detached leaves and their subsequent culture under ex vitro conditions. We demonstrated that hairy roots derived from leaves of two (tropical and temperate) soybean cultivars could be successfully infected by economically important species of root-knot nematodes (Meloidogyne incognita and M. javanica). The established detached-leaf method was further explored for functional validation of two candidate genes encoding for cell wall modifying proteins (CWMPs) to promote resistance against M. incognita through distinct biotechnological strategies: the overexpression of a wild Arachis α-expansin transgene (AdEXPA24) and the dsRNA-mediated silencing of an endogenous soybean polygalacturonase gene (GmPG). AdEXPA24 overexpression in hairy roots of RKN-susceptible soybean cultivar significantly reduced nematode infection by approximately 47%, whereas GmPG downregulation caused an average decrease of 37%. This novel system of hairy root induction from detached leaves showed to be an efficient, practical, fast, and low-cost method suitable for high throughput in root analysis of candidate genes in soybean.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glycine max / Nematoides Tipo de estudo: Screening_studies Limite: Animals Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Glycine max / Nematoides Tipo de estudo: Screening_studies Limite: Animals Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos