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Nano-antibiotic based on silver nanoparticles functionalized to the vancomycin-cysteamine complex for treating Staphylococcus aureus and Enterococcus faecalis.
Veriato, Thaís S; Fontoura, Inglid; Oliveira, Luciane D; Raniero, Leandro J; Castilho, Maiara L.
Afiliação
  • Veriato TS; Bionanotechnology Laboratory, Research & Development Institute, University of Vale do Paraíba, São José dos Campos, São Paulo, 12244000, Brazil.
  • Fontoura I; Bionanotechnology Laboratory, Research & Development Institute, University of Vale do Paraíba, São José dos Campos, São Paulo, 12244000, Brazil.
  • Oliveira LD; Department of Biosciences and Oral Diagnosis, Institute of Science and Technology, Sao Paulo State University, São José dos Campos, São Paulo, 12245-000, Brazil.
  • Raniero LJ; Nanosensors Laboratory, Research & Development Institute, University of Vale do Paraíba, São José dos Campos, São Paulo, 12244000, Brazil.
  • Castilho ML; Bionanotechnology Laboratory, Research & Development Institute, University of Vale do Paraíba, São José dos Campos, São Paulo, 12244000, Brazil. mcastilho@univap.br.
Pharmacol Rep ; 75(4): 951-961, 2023 Aug.
Article em En | MEDLINE | ID: mdl-37171518
BACKGROUND: Bacterial resistance is defined as a microorganism's capacity to develop mechanisms for resisting a determined antimicrobial. Gram-positive bacteria, such as Staphylococcus aureus (S. aureus) and Enterococcus faecalis (E. faecalis), are internationally recognized among the isolates with this resistance profile. In this context, the demand for new medicines has risen, and silver nanoparticles (AgNPs) have been highlighted, especially for their anti-bacterial effects. To develop a nano-antibiotic for treating these Gram-positive strains, we herein report synthesizing and characterizing a nano-antibiotic based on AgNPs functionalized with the complex vancomycin-cysteamine. METHODS: AgNPs were produced using the bottom-up methodology and functionalized with vancomycin modified by the carbodiimide chemistry, forming Ag@vancomycin. Susceptibility tests were performed using S. aureus and E. faecalis strains to assess the bacteriostatic and bactericidal potential of the developed nano-antibiotic. RESULTS: Fourier transform infrared spectroscopy measurements showed the efficacy of vancomycin chemical modification, and the characteristic bands of AgNPs functionalization with the antibiotic. The increase in the nano-antibiotic average hydrodynamic diameter observed by dynamic light scattering proved the presence of vancomycin at the surface of AgNPs. The data from the minimum inhibitory concentration and minimal bactericidal concentration assays tested on standard and clinical planktonic strains of S. aureus and E. faecalis presented excellent performance. CONCLUSION: The results indicate the promising development of a new nano-antibiotic in which the functionalization potentiates the bacteriostatic action of AgNPs and vancomycin with greater efficacy against Gram-positive strains.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nanopartículas Metálicas / Antibacterianos Idioma: En Revista: Pharmacol Rep Assunto da revista: FARMACOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nanopartículas Metálicas / Antibacterianos Idioma: En Revista: Pharmacol Rep Assunto da revista: FARMACOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça