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Characterization of high-yield Bacillus subtilis cysteine protease for diverse industrial applications.
Keshapaga, Usha Rani; Jathoth, Kalyani; Singh, Surya Satyanarayana; Gogada, Raghu; Burgula, Sandeepta.
Afiliação
  • Keshapaga UR; Department of Microbiology, University College of Science, Osmania University, Tarnaka, Hyderabad, 500 007, Telangana, India.
  • Jathoth K; Department of Microbiology, University College of Science, Osmania University, Tarnaka, Hyderabad, 500 007, Telangana, India.
  • Singh SS; Department of Biochemistry , University College of Science, Osmania University , Tarnaka, Hyderabad, 500 007, Telangana, India.
  • Gogada R; Department of Biochemistry , University College of Science, Osmania University , Tarnaka, Hyderabad, 500 007, Telangana, India.
  • Burgula S; Department of Microbiology, University College of Science, Osmania University, Tarnaka, Hyderabad, 500 007, Telangana, India. s_burgula@osmania.ac.in.
Braz J Microbiol ; 54(2): 739-752, 2023 Jun.
Article em En | MEDLINE | ID: mdl-37157054
Bacterial proteases have extensive applications in various fields of industrial microbiology. In this study, protease-producing organisms were screened on skimmed milk agar media using serial dilution. Through microbial biomass production, biochemical tests, protease-specific activity, and 16 s RNA gene sequencing, the isolates were identified as Bacillus subtilis and submitted to NCBI. The strain accession numbers were designated as A1 (MT903972), A2 (MT903996), A4 (MT904091), and A5 (MT904796). The strain A4 Bacillus subtilis showed highest protease-specific activity as 76,153.84 U/mg. A4 Bacillus subtilis was unaffected by Ca2+, Cu2+, Fe2+, Hg2+, Mg2+, Na, Fe2+, and Zn2+ but was inhibited by 80% by Mn2+ (5 mM). The protease activity was inhibited by up to 30% by iodoacetamide (5 mM). These findings confirm the enzyme to be a cysteine protease which was further confirmed by MALDI-TOF. The identified protease showed 71% sequence similarity with Bacillus subtilis cysteine protease. The crude cysteine protease significantly aided in fabric stain removal when added to a generic detergent. It also aided in the recovery of silver from used X-ray films and de-hairing of goat skin hides and showed decent application in meat tenderization. Thus, the isolated cysteine protease has high potential for industrial applications.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacillus subtilis / Cisteína Proteases Tipo de estudo: Prognostic_studies Idioma: En Revista: Braz J Microbiol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Índia País de publicação: Brasil
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Bacillus subtilis / Cisteína Proteases Tipo de estudo: Prognostic_studies Idioma: En Revista: Braz J Microbiol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Índia País de publicação: Brasil