Your browser doesn't support javascript.
loading
Naringenin and proanthocyanidins pre-treatment decreases synthesis and activity of gelatinases induced by zoledronic acid in a dental implant surface in vitro model.
Cardoso, Lais Medeiros; Pansani, Taisa Nogueira; de Souza Costa, Carlos Alberto; Basso, Fernanda Gonçalves.
Afiliação
  • Cardoso LM; Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP) - School of Dentistry, 1680 Humaitá Street, Araraquara, São Paulo 14801-903, Brazil.
  • Pansani TN; Department of Dental Materials and Prosthodontics, São Paulo State University (UNESP) - School of Dentistry, 1680 Humaitá Street, Araraquara, São Paulo 14801-903, Brazil.
  • de Souza Costa CA; Department of Physiology and Pathology, São Paulo State University (UNESP) - School of Dentistry, 1680 Humaitá Street, Araraquara, São Paulo 14801-903, Brazil.
  • Basso FG; Department of Dentistry, Ribeirão Preto University (UNAERP), 2201 Costábile Romano Avenue, Ribeirão Preto, São Paulo 14096-900, Brazil. Electronic address: fergbasso@gmail.com.
Arch Oral Biol ; 151: 105703, 2023 Jul.
Article em En | MEDLINE | ID: mdl-37146390
OBJECTIVE: To assess the effects of pre-treatment with proanthocyanidins (PA) flavonoids, from grape seed extract, and synthetic naringenin (NA) on the synthesis of matrix metalloproteinases (MMPs) gelatinases and their tissue inhibitors (TIMPs), as well as the gelatinolytic activity of MMPs by human gingival fibroblasts (HGF) and osteoblasts (Ob) exposed to zoledronic acid (ZA) in a dental implant surface in vitro model. DESIGN: The highest non-cytotoxic concentrations of NA and PA were determined for HGF (10 µg/mL; defined by previous study) and Ob (0.5 µg/mL; defined by prestoBlue assay). Then, HFG and Ob were individually seeded onto titanium discs, and after 24 h, cells were pre-treated (or not) with NA or PA, followed (or not) by exposure to ZA. Next, MMP-2, MMP-9, TIMP-1, TIMP-2 synthesis (ELISA), and gelatinolytic activity (in situ zymography) was evaluated. Data were analyzed by one-way ANOVA and Tukey tests (α = 0.05). RESULTS: ZA treatment increased the synthesis (p < 0.05) and activity of MMPs; flavonoids pre-treatment controlled ZA-induced gelatinolytic effects, down-regulating MMPs synthesis (p < 0.05) and activity by HGF and Ob. For HGF, NA and PA pre-treatment did not up-regulate TIMP synthesis after ZA exposure (p > 0.05); for Ob, TIMP-2 was up-regulated (p < 0.05) by flavonoids, followed by ZA. CONCLUSIONS: NA and PA pre-treatment provides interesting results in the modulation of ZA deleterious effects, down-regulating MMP-2 and MMP-9 synthesis and activity by HGF and Ob and up-regulating TIMP-2 by Ob.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Implantes Dentários / Proantocianidinas Limite: Humans Idioma: En Revista: Arch Oral Biol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Implantes Dentários / Proantocianidinas Limite: Humans Idioma: En Revista: Arch Oral Biol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido