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l-carvone decreases breast cancer cells adhesion, migration, and invasion by suppressing FAK activation.
Lima, Lucas Trevisan França de; Ganzella, Fernando Augusto de Oliveira; Cardoso, Gabriela Casani; Pires, Verônica Dos Santos; Chequin, Andressa; Santos, Giulia Luiza; Braun-Prado, Karin; Galindo, Claudia Martins; Braz Junior, Odair; Molento, Marcelo Beltrão; Acco, Alexandra; Adami, Eliana Rezende; Costa, Erico Tosoni; Cavichiolo Franco, Célia Regina; Klassen, Giseli; Ramos, Edneia Amancio de Souza.
Afiliação
  • Lima LTF; Pos-graduate Program of Microbiology, Parasitology and Pathology, Federal University of Parana, Curitiba, PR, Brazil.
  • Ganzella FAO; Department of Basic Pathology, Federal University of Parana, Curitiba, PR, Brazil.
  • Cardoso GC; Pos-graduate Program of Microbiology, Parasitology and Pathology, Federal University of Parana, Curitiba, PR, Brazil.
  • Pires VDS; Pos-graduate Program of Microbiology, Parasitology and Pathology, Federal University of Parana, Curitiba, PR, Brazil.
  • Chequin A; Pos-graduate Program of Microbiology, Parasitology and Pathology, Federal University of Parana, Curitiba, PR, Brazil.
  • Santos GL; Molecular Oncology Center, Research and Education Institute, Hospital Sirio-Libanes, São Paulo, SP, Brazil.
  • Braun-Prado K; Department of Basic Pathology, Federal University of Parana, Curitiba, PR, Brazil.
  • Galindo CM; Pos-graduate Program of Pharmacology, Federal University of Parana, Curitiba, PR, Brazil.
  • Braz Junior O; Pos-graduate Program of Cellular and Molecular Biology, Federal University of Parana, Curitiba, PR, Brazil.
  • Molento MB; Pos-graduate Program of Microbiology, Parasitology and Pathology, Federal University of Parana, Curitiba, PR, Brazil; Department of Veterinary Medicine, Federal University of Parana, Curitiba, PR, Brazil.
  • Acco A; Pos-graduate Program of Pharmacology, Federal University of Parana, Curitiba, PR, Brazil.
  • Adami ER; Pos-graduate Program of Pharmacology, Federal University of Parana, Curitiba, PR, Brazil.
  • Costa ET; Molecular Oncology Center, Research and Education Institute, Hospital Sirio-Libanes, São Paulo, SP, Brazil.
  • Cavichiolo Franco CR; Pos-graduate Program of Cellular and Molecular Biology, Federal University of Parana, Curitiba, PR, Brazil.
  • Klassen G; Pos-graduate Program of Microbiology, Parasitology and Pathology, Federal University of Parana, Curitiba, PR, Brazil; Department of Basic Pathology, Federal University of Parana, Curitiba, PR, Brazil.
  • Ramos EAS; Pos-graduate Program of Microbiology, Parasitology and Pathology, Federal University of Parana, Curitiba, PR, Brazil; Department of Basic Pathology, Federal University of Parana, Curitiba, PR, Brazil. Electronic address: edneiaama@ufpr.br.
Chem Biol Interact ; 378: 110480, 2023 Jun 01.
Article em En | MEDLINE | ID: mdl-37059214
Breast cancer is one of the most common types of cancer in the world and current therapeutic strategies present severe drawbacks. l-carvone (CRV), a monoterpene found in Mentha spicata (spearmint), has been reported to have potent anti-inflammatory activity. Here, we examined the role of CRV in breast cancer cell adhesion, migration and invasion in vitro and how this component could suppress the growth of Ehrlich carcinoma-bearing mice. In vivo, treatment with CRV significantly decreased tumor growth, increased tumor necrosis area, and reduced the expression of VEGF and HIF-1α in Ehrlich carcinoma-bearing mice. Furthermore, the anticancer efficacy of CRV was similar to currently used chemotherapy (Methotrexate), and the combination of CRV with MTX potentiated the chemotherapy effects. Further mechanistic investigation in vitro revealed that CRV modulates the interaction of breast cancer cells with the extracellular matrix (ECM) by disrupting focal adhesion, which was shown by scanning electron microscopy (SEM) and immunofluorescence. Moreover, CRV caused a decrease in ß1-integrin expression and inhibited focal adhesion kinase (FAK) activation. FAK is one of the most important downstream activators of several metastatic processes, including MMP-2 mediated invasion and HIF-1α/VEGF angiogenesis stimulus, both of which were found to be reduced in MDA-MB-231 cells exposed to CRV. Our results provide new insight about targeting ß1-integrin/FAK signaling pathway with CRV, which could be a new potential agent in the treatment of breast cancer.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carcinoma / Fator A de Crescimento do Endotélio Vascular Limite: Animals Idioma: En Revista: Chem Biol Interact Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Irlanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Carcinoma / Fator A de Crescimento do Endotélio Vascular Limite: Animals Idioma: En Revista: Chem Biol Interact Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Irlanda