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Melanopsin (OPN4) is a novel player in skin homeostasis and attenuates UVA-induced effects.
Sua-Cespedes, Cristhian; Lacerda, José Thalles; Zanetti, Giovanna; David, Daniela Dantas; Moraes, Maria Nathalia; de Assis, Leonardo V M; Castrucci, Ana Maria L.
Afiliação
  • Sua-Cespedes C; Department of Physiology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.
  • Lacerda JT; Department of Physiology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.
  • Zanetti G; Department of Physiology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.
  • David DD; Department of Physiology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil.
  • Moraes MN; Department of Biological Sciences, Institute of Environmental, Chemical and Pharmaceutical Sciences, Federal University of São Paulo, São Paulo, Brazil.
  • de Assis LVM; Institute of Neurobiology, University of Lübeck, Lübeck, Germany.
  • Castrucci AML; Department of Physiology, Institute of Biosciences, University of São Paulo, São Paulo, Brazil; Department of Biology, University of Virginia, Charlottesville, USA. Electronic address: amdlcast@ib.usp.br.
J Photochem Photobiol B ; 242: 112702, 2023 May.
Article em En | MEDLINE | ID: mdl-37018912
The presence of melanopsin (OPN4) has been shown in cultured murine melanocytes and was associated with ultraviolet A radiation (UVA) reception. Here we demonstrated the protective role of OPN4 in skin physiology and the increased UVA-induced damage in its absence. Histological analysis showed a thicker dermis and thinner hypodermal white adipose tissue layer in Opn4-/- (KO) mice than in wild-type (WT) animals. Proteomics analyses revealed molecular signatures associated with proteolysis, remodeling chromatin, DNA damage response (DDR), immune response, and oxidative stress coupled with antioxidant responses in the skin of Opn4 KO mice compared to WT. Skin protein variants were found in Opn4 KO mice and Opn2, Opn3, and Opn5 gene expressions were increased in the genotype. We investigated each genotype response to UVA stimulus (100 kJ/m2). We found an increase of Opn4 gene expression following stimulus on the skin of WT mice suggesting melanopsin as a UVA sensor. Proteomics findings suggest that UVA decreases DDR pathways associated with ROS accumulation and lipid peroxidation in the skin of Opn4 KO mice. Relative changes in methylation (H3-K79) and acetylation sites of histone between genotypes and differentially modulated by UVA stimulus were also observed. We also identified alterations of molecular traits of the central hypothalamus-pituitary- adrenal (HPA) and the skin HPA-like axes in the absence of OPN4. Higher skin corticosterone levels were detected in UVA-stimulated Opn4 KO compared to irradiated WT mice. Taken altogether, functional proteomics associated with gene expression experiments allowed a high-throughput evaluation that suggests an important protective role of OPN4 in regulating skin physiology in the presence and absence of UVA radiation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pele / Opsinas de Bastonetes Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Photochem Photobiol B Assunto da revista: BIOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pele / Opsinas de Bastonetes Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: J Photochem Photobiol B Assunto da revista: BIOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça