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Spleen regeneration after subcutaneous heterotopic autotransplantation in a mouse model.
Elchaninov, Andrey; Vishnyakova, Polina; Lokhonina, Anastasiya; Kiseleva, Viktoria; Menyailo, Egor; Antonova, Maria; Mamedov, Aiaz; Arutyunyan, Irina; Bolshakova, Galina; Goldshtein, Dmitry; Bao, Xuhui; Fatkhudinov, Timur; Sukhikh, Gennady.
Afiliação
  • Elchaninov A; Laboratory of Growth and Development, Avtsyn Research Institute of Human Morphology of FSBI Petrovsky National Research Centre of Surgery, Moscow, Russia. elchandrey@yandex.ru.
  • Vishnyakova P; Histology Department, Medical Institute, Peoples' Friendship University of Russia (RUDN University), Moscow, Russia. elchandrey@yandex.ru.
  • Lokhonina A; Laboratory of Regenerative Medicine, National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, Moscow, Russia.
  • Kiseleva V; Histology Department, Medical Institute, Peoples' Friendship University of Russia (RUDN University), Moscow, Russia.
  • Menyailo E; Laboratory of Regenerative Medicine, National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, Moscow, Russia.
  • Antonova M; Histology Department, Medical Institute, Peoples' Friendship University of Russia (RUDN University), Moscow, Russia.
  • Mamedov A; Laboratory of Regenerative Medicine, National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, Moscow, Russia.
  • Arutyunyan I; Laboratory of Growth and Development, Avtsyn Research Institute of Human Morphology of FSBI Petrovsky National Research Centre of Surgery, Moscow, Russia.
  • Bolshakova G; Histology Department, Pirogov Russian National Research Medical University, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
  • Goldshtein D; Histology Department, Pirogov Russian National Research Medical University, Ministry of Healthcare of the Russian Federation, Moscow, Russia.
  • Bao X; Laboratory of Regenerative Medicine, National Medical Research Center for Obstetrics, Gynecology and Perinatology Named after Academician V.I. Kulakov of Ministry of Healthcare of Russian Federation, Moscow, Russia.
  • Fatkhudinov T; Laboratory of Growth and Development, Avtsyn Research Institute of Human Morphology of FSBI Petrovsky National Research Centre of Surgery, Moscow, Russia.
  • Sukhikh G; Laboratory of Stem Cells Genetics, Research Center of Medical Genetics, Moscow, Russia.
Biol Res ; 56(1): 15, 2023 Mar 29.
Article em En | MEDLINE | ID: mdl-36991509
BACKGROUND: Splenectomy may lead to severe postoperative complications, including sepsis and cancers. A possible solution to this problem is heterotopic autotransplantation of the spleen. Splenic autografts rapidly restore the regular splenic microanatomy in model animals. However, the functional competence of such regenerated autografts in terms of lympho- and hematopoietic capacity remains uncertain. Therefore, this study aimed to monitor the dynamics of B and T lymphocyte populations, the monocyte-macrophage system, and megakaryocytopoiesis in murine splenic autografts. METHODS: The model of subcutaneous splenic engraftment was implemented in C57Bl male mice. Cell sources of functional recovery were studied using heterotopic transplantations from B10-GFP donors to C57Bl recipients. The cellular composition dynamics were studied by immunohistochemistry and flow cytometry. Expression of regulatory genes at mRNA and protein levels was assessed by real-time PCR and Western blot, respectively. RESULTS: Characteristic splenic architecture is restored within 30 days post-transplantation, consistent with other studies. The monocyte-macrophage system, megakaryocytes, and B lymphocytes show the highest rates, whereas the functional recovery of T cells takes longer. Cross-strain splenic engraftments using B10-GFP donors indicate the recipient-derived cell sources of the recovery. Transplantations of scaffolds populated with splenic stromal cells or without them afforded no restoration of the characteristic splenic architecture. CONCLUSIONS: Allogeneic subcutaneous transplantation of splenic fragments in a mouse model leads to their structural recovery within 30 days, with full reconstitution of the monocyte-macrophage, megakaryocyte and B lymphocyte populations. The circulating hematopoietic cells provide the likely source for the cell composition recovery.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Baço / Esplenectomia Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Biol Res Assunto da revista: BIOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Federação Russa País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Baço / Esplenectomia Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Biol Res Assunto da revista: BIOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Federação Russa País de publicação: Reino Unido