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Characterization and Expression of Holothurian Wnt Signaling Genes during Adult Intestinal Organogenesis.
Auger, Noah A; Medina-Feliciano, Joshua G; Quispe-Parra, David J; Colón-Marrero, Stephanie; Ortiz-Zuazaga, Humberto; García-Arrarás, José E.
Afiliação
  • Auger NA; Department of Biology, University of Puerto Rico, Rio Piedras Campus, San Juan 00925, Puerto Rico.
  • Medina-Feliciano JG; Department of Biology, University of Puerto Rico, Rio Piedras Campus, San Juan 00925, Puerto Rico.
  • Quispe-Parra DJ; Department of Biology, University of Puerto Rico, Rio Piedras Campus, San Juan 00925, Puerto Rico.
  • Colón-Marrero S; Department of Biology, University of Puerto Rico, Rio Piedras Campus, San Juan 00925, Puerto Rico.
  • Ortiz-Zuazaga H; Department of Computer Science, University of Puerto Rico, Rio Piedras Campus, San Juan 00925, Puerto Rico.
  • García-Arrarás JE; Department of Biology, University of Puerto Rico, Rio Piedras Campus, San Juan 00925, Puerto Rico.
Genes (Basel) ; 14(2)2023 01 25.
Article em En | MEDLINE | ID: mdl-36833237
Wnt signaling has been shown to play multiple roles in regenerative processes, one of the most widely studied of which is the regeneration of the intestinal luminal epithelia. Most studies in this area have focused on self-renewal of the luminal stem cells; however, Wnt signaling may also have more dynamic functions, such as facilitating intestinal organogenesis. To explore this possibility, we employed the sea cucumber Holothuria glaberrima that can regenerate a full intestine over the course of 21 days after evisceration. We collected RNA-seq data from various intestinal tissues and regeneration stages and used these data to define the Wnt genes present in H. glaberrima and the differential gene expression (DGE) patterns during the regenerative process. Twelve Wnt genes were found, and their presence was confirmed in the draft genome of H. glaberrima. The expressions of additional Wnt-associated genes, such as Frizzled and Disheveled, as well as genes from the Wnt/ß-catenin and Wnt/Planar Cell Polarity (PCP) pathways, were also analyzed. DGE showed unique distributions of Wnt in early- and late-stage intestinal regenerates, consistent with the Wnt/ß-catenin pathway being upregulated during early-stages and the Wnt/PCP pathway being upregulated during late-stages. Our results demonstrate the diversity of Wnt signaling during intestinal regeneration, highlighting possible roles in adult organogenesis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Beta Catenina / Via de Sinalização Wnt Limite: Animals Idioma: En Revista: Genes (Basel) Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Porto Rico País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Beta Catenina / Via de Sinalização Wnt Limite: Animals Idioma: En Revista: Genes (Basel) Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Porto Rico País de publicação: Suíça