Evaluation of Early Reading of Broth Microdilution Technique for Polymyxin B.

Carneiro, Maiara Dos Santos; Volpato, Fabiana Caroline Zempulski; Wilhelm, Camila Mörschbächer; Wink, Priscila Lamb; Barth, Afonso Luís

Carneiro, Maiara Dos Santos; Volpato, Fabiana Caroline Zempulski; Wilhelm, Camila Mörschbächer; Wink, Priscila Lamb; Barth, Afonso Luís.

Afiliação

Carneiro MDS; LABRESIS-Laboratório de Pesquisa em Resistência Bacteriana, Hospital de Clínicas de Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil.
Volpato FCZ; PPGCF-Programa de Pós-Graduação em Ciências Farmacêuticas, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Wilhelm CM; LABRESIS-Laboratório de Pesquisa em Resistência Bacteriana, Hospital de Clínicas de Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil.
Wink PL; PPGCM-Programa de Pós-Graduação em Ciências Médicas, Faculdade de Medicina, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, Brazil.
Barth AL; LABRESIS-Laboratório de Pesquisa em Resistência Bacteriana, Hospital de Clínicas de Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil.

Microb Drug Resist ; 29(2): 59-64, 2023 Feb.

Article em En | MEDLINE | ID: mdl-36802271

RESUMO

Delay in the results of standard phenotypic susceptibility tests is the main obstacle to adequate antibiotic treatment. For this reason, the European Committee for Antimicrobial Susceptibility Testing has proposed the Rapid Antimicrobial Susceptibility Testing for the disk diffusion method directly from blood culture. However, to date, there are no studies evaluating early readings of polymyxin B broth microdilution (BMD), the only standardized methodology for assessing susceptibility to polymyxins. This study aimed to evaluate modifications in the BMD technique for polymyxin B using fewer antibiotic dilutions and reading after an incubation time of 8-9 hr (early reading) in comparison to 16-20 hr of incubation (standard reading) for isolates of Enterobacterales, Acinetobacter baumannii complex, and Pseudomonas aeruginosa. A total of 192 isolates of gram-negative bacteria were evaluated and the minimum inhibitory concentrations were read after early and standard incubations. The early reading presented 93.2% of essential agreement and 97.9% of categorical agreement with the standard reading of BMD. Only three isolates (2.2%) presented major errors and only one (1.7%) presented a very major error. These results indicate a high agreement between the early and the standard reading times of BMD of polymyxin B.

Assuntos

Antibacterianos; Polimixina B; Polimixina B/farmacologia; Antibacterianos/farmacologia; Colistina/farmacologia; Testes de Sensibilidade Microbiana; Polimixinas

Palavras-chave

Rapid Antimicrobial Susceptibility Testing; broth microdilution; early reading; gram-negative bacteria; minimum inhibitory concentrations; polymyxin B

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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Polimixina B / Antibacterianos Idioma: En Revista: Microb Drug Resist Assunto da revista: MICROBIOLOGIA / TERAPIA POR MEDICAMENTOS Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos

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