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Synthesis of Submicrometric Chitosan Particles Loaded with Calcium Phosphate for Biomedical Applications.
Lopes, Diana Pereira; Freitas, Selma Regina Muniz; Tanaka, Carina Baptiston; Delechiave, Giovanne; Kikuchi, Lucia Nobuco Takamori; Braga, Roberto R; Kruzic, Jamie J; Moreira, Maria Stella; Boaro, Leticia Cristina Cidreira; Catalani, Luiz Henrique; Gonçalves, Flávia.
Afiliação
  • Lopes DP; Departamento de Odontologia, Universidade Ibirapuera, Av. Interlagos 1329 - 4° andar, São Paulo, SP, 04661-100, Brazil.
  • Freitas SRM; Faculdade de Odontologia, Universidade Santo Amaro, Av. Prof. Eneas de Siqueira Neto, 340, São Paulo, SP, 04829-900, Brazil.
  • Tanaka CB; Centre for Rural Dentistry & Oral Health, Charles Sturt University, Orange, NSW, 2800, Australia.
  • Delechiave G; Departamento de Química Fundamental, Instituto de Química da Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, São Paulo, SP, 05508-000, Brazil.
  • Kikuchi LNT; Departamento de Odontologia, Universidade Ibirapuera, Av. Interlagos 1329 - 4° andar, São Paulo, SP, 04661-100, Brazil.
  • Braga RR; Faculdade de Odontologia da Universidade de São Paulo, Departamento de Biomateriais e Biologia Oral, Av. Prof. Lineu Prestes, 2222, São Paulo, SP, 05508-000, Brazil.
  • Kruzic JJ; School of Mechanical and Manufacturing Engineering, University of New South Wales (UNSW Sydney), Sydney, NSW, 2052, Australia.
  • Moreira MS; Departamento de Odontologia, Universidade Ibirapuera, Av. Interlagos 1329 - 4° andar, São Paulo, SP, 04661-100, Brazil.
  • Boaro LCC; Faculdade de Odontologia, Universidade Santo Amaro, Av. Prof. Eneas de Siqueira Neto, 340, São Paulo, SP, 04829-900, Brazil. leticiacidreiraboaro@gmail.com.
  • Catalani LH; Departamento de Química Fundamental, Instituto de Química da Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, São Paulo, SP, 05508-000, Brazil.
  • Gonçalves F; Faculdade de Odontologia, Universidade Santo Amaro, Av. Prof. Eneas de Siqueira Neto, 340, São Paulo, SP, 04829-900, Brazil.
AAPS PharmSciTech ; 24(2): 56, 2023 Feb 09.
Article em En | MEDLINE | ID: mdl-36759364
Chitosan particles loaded with dibasic calcium phosphate anhydrous (DCPA) is a promising strategy for combining antimicrobial and osteoconduction properties in regenerative medicine. However, mostly micrometer-sized particles have been reported in the literature, limiting their use and reducing their effect in the biomedical field. We have recently overcome this limitation by developing submicrometer-sized particles with electrospray technique. The objective of this study was to understand how the process parameters control the size and properties of submicrometer chitosan particles loaded with DCPA. Solutions of 10 mg/mL chitosan and 2.5 mg/mL DCPA in a 90% acetic acid were electrosprayed under three distinct flow rate conditions: 0.2, 0.5, and 1.0 mL/h. The particles were crosslinked in a glutaraldehyde atmosphere and characterized in terms of their morphology, inorganic content, zeta potential, and minimum inhibitory concentration (MIC) against S. mutans. All conditions showed particles with two similar morphologies: one small-sized with a spherical shape and another larger-sized with a bi-concave shape. All generated a broad particle size distribution, with a similar mean size of ~ 235 nm. The addition of DCPA decreased the zeta potential for all the samples, but it was above 30 mV, indicating a low aggregation potential. The lower flow rate showed the worst efficacy for DCPA incorporation. Antimicrobial activity was greater in chitosan/DCPA particles with flow rate of 0.5 mL/h. It can be concluded that the flow rate of 0.5 mL/h presents the best compromise solution in terms of morphology, zeta potential, MIC, and inorganic content.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Quitosana / Anti-Infecciosos Idioma: En Revista: AAPS PharmSciTech Assunto da revista: FARMACOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Quitosana / Anti-Infecciosos Idioma: En Revista: AAPS PharmSciTech Assunto da revista: FARMACOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos