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Dry tobacco leaves: an in vivo and in silico approach to the consequences of occupational exposure.
Dalberto, Daiana; Garcia, Ana L H; De Souza, Melissa R; Picinini, Juliana; Soares, Solange; De Souza, Guilherme M S; Chytry, Paola; Dias, Johnny F; Salvador, Mirian; Da Silva, Fernanda R; Da Silva, Juliana.
Afiliação
  • Dalberto D; Laboratory of Genetic Toxicology, PPGBioSaúde, Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil.
  • Garcia ALH; Laboratory of Genetic Toxicology, PPGBioSaúde, Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil.
  • De Souza MR; Laboratory of Genetic Toxicology, PPGSDH, La Salle University (UniLaSalle), Canoas, RS, Brazil.
  • Picinini J; Laboratory of Genetic Toxicology, PPGBioSaúde, Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil.
  • Soares S; Laboratory of Genetic Toxicology, PPGBioSaúde, Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil.
  • De Souza GMS; Laboratory of Genetic Toxicology, PPGBioSaúde, Lutheran University of Brazil (ULBRA), Canoas, RS, Brazil.
  • Chytry P; Ion Implantation Laboratory, Institute of Physics, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
  • Dias JF; Ion Implantation Laboratory, Institute of Physics, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
  • Salvador M; Ion Implantation Laboratory, Institute of Physics, Federal University of Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil.
  • Da Silva FR; Biotechnology Institute, University of Caxias do Sul, Caxias do Sul (UCS), RS, Brazil.
  • Da Silva J; Laboratory of Genetic Toxicology, PPGSDH, La Salle University (UniLaSalle), Canoas, RS, Brazil.
Mutagenesis ; 38(2): 120-130, 2023 05 12.
Article em En | MEDLINE | ID: mdl-36738258
Exposure of tobacco workers handling dried tobacco leaves has been linked to an increased risk of toxicity and respiratory illness due to the presence of nicotine and other chemicals. This study aimed to evaluate the DNA damage caused by the exposure of tobacco growers during the dry leaf classification process and the relation to cellular mechanisms. A total of 86 individuals participated in the study, divided into a group exposed to dry tobacco (n = 44) and a control group (n = 42). Genotoxicity was evaluated using the alkaline comet assay and lymphocyte micronucleus (MN) assay (CBMN-Cyt), and measurement of telomere length. The levels of oxidative and nitrosative stress were evaluated through the formation of thiobarbituric acid reactive species, and nitric oxide levels, respectively. The inorganic elements were measured in the samples using particle-induced X-ray emission method. The combination of variables was demonstrated through principal component analysis and the interactions were expanded through systems biology. Comet assay, MN, death cells, thiobarbituric acid reactive species, and nitrosative stress showed a significant increase for all exposed groups in relation to the control. Telomere length showed a significant decrease for exposed women and total exposed group in relation to men and control groups, respectively. Bromine (Br) and rubidium (Rb) in the exposed group presented higher levels than control groups. Correlations between nitrate and apoptosis; Br and MN and necrosis; and Rb and telomeres; besides age and DNA damage and death cells were observed. The systems biology analysis demonstrated that tobacco elements can increase the nuclear translocation of NFKB dimers inducing HDAC2 expression, which, associated with BRCA1 protein, can potentially repress transcription of genes that promote DNA repair. Dry tobacco workers exposed to dry leaves and their different agents showed DNA damage by different mechanisms, including redox imbalance.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nicotiana / Exposição Ocupacional Limite: Female / Humans / Male Idioma: En Revista: Mutagenesis Assunto da revista: GENETICA MEDICA / SAUDE AMBIENTAL Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Nicotiana / Exposição Ocupacional Limite: Female / Humans / Male Idioma: En Revista: Mutagenesis Assunto da revista: GENETICA MEDICA / SAUDE AMBIENTAL Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido