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Diagnosis and phylogenetic analysis of bovine leukemia virus in dairy cattle in northeastern Brazil.
Pereira, José Gomes; Silva, Cândida de Assunção; Silva, Lucas Diniz; Lima, Cristian Alex Aquino; do Rosário, Carla Janaina Rebouças Marques; Silva, Ellainy Maria Conceição; Oliveira, Maria do Socorro Costa; Ribeiro, Larissa Sarmento Dos Santos; Santos, Hamilton Pereira; Abreu-Silva, Ana Lucia; Melo, Ferdinan Almeida.
Afiliação
  • Pereira JG; Department of Pathology, State University of Maranhão, São Luís, Brazil.
  • Silva CA; Department of Pathology, State University of Maranhão, São Luís, Brazil.
  • Silva LD; Department of Pathology, State University of Maranhão, São Luís, Brazil.
  • Lima CAA; Department of Pathology, State University of Maranhão, São Luís, Brazil.
  • do Rosário CJRM; Department of Pathology, State University of Maranhão, São Luís, Brazil.
  • Silva EMC; Department of Pathology, State University of Maranhão, São Luís, Brazil.
  • Oliveira MDSC; Department of Pathology, State University of Maranhão, São Luís, Brazil.
  • Ribeiro LSDS; Department of Pathology, State University of Maranhão, São Luís, Brazil.
  • Santos HP; Department of Pathology, State University of Maranhão, São Luís, Brazil.
  • Abreu-Silva AL; Department of Pathology, State University of Maranhão, São Luís, Brazil.
  • Melo FA; Department of Pathology, State University of Maranhão, São Luís, Brazil.
Front Vet Sci ; 9: 1080994, 2022.
Article em En | MEDLINE | ID: mdl-36713884
Enzootic bovine leukosis (EBL) is a chronic viral disease of wide distribution in cattle herds and may take several years for the first manifestation of clinical signs. Most animals do not present clinical signs. However, the economic losses are underestimated due to this disease. Thus, this work aimed to detect and characterize BLV in dairy cattle in the Maranhão state, northeastern Brazil. Blood samples were collected from 176 animals from 8 municipalities in the southeastern state of Maranhão. Bovine blood samples were subjected to DNA extraction and molecular diagnosis using nested PCR assays for BLV, targeting gp51 gene. Positive samples were then sequenced and then subjected to phylogenetic inferences. BLV DNA was detected in 16 cattle (16/176, 9.09%) in 4 municipalities. Phylogenetic analyzes showed that the sequence obtained clustered in a clade containing BLV sequences classified as genotype 6, with a high degree of support. Our data shows BLV occurrence in the Northeast of Brazil and the identification of genotype 6 in this region. These findings contribute to the molecular epidemiology of this agent in Brazil.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Prognostic_studies País/Região como assunto: America do sul / Brasil Idioma: En Revista: Front Vet Sci Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Tipo de estudo: Diagnostic_studies / Prognostic_studies País/Região como assunto: America do sul / Brasil Idioma: En Revista: Front Vet Sci Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça