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Performance of Bleach Method Sputum Smear Microscopy for the Diagnosis of Tuberculosis in a Highly Endemic District in Lima, Peru.
Rosales-Rimache, Jaime; Nunayalle-Vargas, Magda; Rueda-Torres, Lenin; Inolopú-Cucche, Jorge.
Afiliação
  • Rosales-Rimache J; Facultad de Medicina, Universidad Privada Norbert Wiener, Lima 39, Peru.
  • Nunayalle-Vargas M; Laboratorio Clínico, Hospital III Suárez Angamos, Essalud, Lima 18, Peru.
  • Rueda-Torres L; Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima 31, Peru.
  • Inolopú-Cucche J; Facultad de Administración y Salud Pública, Universidad Peruana Cayetano Heredia, Lima 31, Peru.
Int J Environ Res Public Health ; 20(1)2022 12 22.
Article em En | MEDLINE | ID: mdl-36612454
Background: Sputum smear microscopy (SSM) is a screening test used to diagnose tuberculosis (TB); however, its performance and sensitivity are relatively low, which can lead to false negatives. We designed a cross-sectional study to estimate the performance of SSM that includes a pretreatment based on sputum digestion with bleach (sodium hypochlorite) for the diagnosis of TB. Methods: We evaluated 73 sputum samples from patients with a diagnosis of TB confirmed by the Xpert MTB/RIF test and 114 samples from patients without TB. We performed sputum digestion using a 5% sodium hypochlorite solution, centrifuged at 2000 rpm for 15 min. We prepared smears for direct and bleach-treated SSM and used Ziehl−Neelsen staining. Results: The bleach-treated SSM obtained absolute identification of the cases of TB confirmed by the Xpert test, compared to 95.9% identified by the direct smear method (without bleach treatment). We also found a significant increase (p < 0.001) in the recovery of acid-fast bacilli (AFB) obtained by the bleach-treated SSM (293.8 ± 215.1 AFB) compared to the direct SSM method (222.9 ± 195.5 AFB). The AUC of the bleach-treated SSM and direct SSM was 100% and 96.6%, respectively. Conclusion: The bleach-treated SSM performs better than the direct SSM in identifying AFB and increasing the bacillary count in the sputum samples.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose / Tuberculose Pulmonar / Mycobacterium tuberculosis Tipo de estudo: Diagnostic_studies / Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Humans País/Região como assunto: America do sul / Peru Idioma: En Revista: Int J Environ Res Public Health Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Peru País de publicação: Suíça
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Tuberculose / Tuberculose Pulmonar / Mycobacterium tuberculosis Tipo de estudo: Diagnostic_studies / Observational_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Humans País/Região como assunto: America do sul / Peru Idioma: En Revista: Int J Environ Res Public Health Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Peru País de publicação: Suíça