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Photo-crosslinked GelMA loaded with dental pulp stem cells and VEGF to repair critical-sized soft tissue defects in rats.
Matheus, Henrique R; Hadad, Henrique; Monteiro, Joao L G C; Takusagawa, Toru; Zhang, Fugui; Ye, Qingsong; He, Yan; Rosales, Ivy A; Jounaidi, Youssef; Randolph, Mark A; Guastaldi, Fernando P S.
Afiliação
  • Matheus HR; Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Harvard School of Dental Medicine, Boston, MA, United States of America; Department of Diagnosis and Surgery, Periodontics Division, São Paulo State University (UNESP), School of Dentistry, Araçatuba, SP, Brazil.
  • Hadad H; Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Harvard School of Dental Medicine, Boston, MA, United States of America; Department of Diagnosis and Surgery, Oral & Maxillofacial Surgery Division, São Paulo State University (UNESP), School of Dentistry, Araçatuba, S
  • Monteiro JLGC; Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Harvard School of Dental Medicine, Boston, MA, United States of America.
  • Takusagawa T; Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Harvard School of Dental Medicine, Boston, MA, United States of America.
  • Zhang F; Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Harvard School of Dental Medicine, Boston, MA, United States of America.
  • Ye Q; Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Harvard School of Dental Medicine, Boston, MA, United States of America.
  • He Y; Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Harvard School of Dental Medicine, Boston, MA, United States of America.
  • Rosales IA; Department of Pathology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States of America.
  • Jounaidi Y; Department of Anaesthesia, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States of America.
  • Randolph MA; Department of Surgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA, United States of America.
  • Guastaldi FPS; Department of Oral and Maxillofacial Surgery, Massachusetts General Hospital, Harvard School of Dental Medicine, Boston, MA, United States of America. Electronic address: fguastaldi@mgh.harvard.edu.
J Stomatol Oral Maxillofac Surg ; 124(1S): 101373, 2023 02.
Article em En | MEDLINE | ID: mdl-36584767
BACKGROUND: Tissue engineering of skin and mucosa is essential for the esthetic and functional reconstruction of individuals disfigured by trauma, resection surgery, or severe burns while overcoming the limited amount of autograft and donor site morbidity. PURPOSE: We aimed to determine whether a combination of Gelatin-methacryloyl (GelMA) hydrogel scaffold alone or loaded with either dental pulp stem cells (DPSCs) and/or vascular endothelial growth factor (VEGF) could improve skin wound healing in rats. MATERIALS AND METHODS: Four 10 mm full-thickness skin defects were created on the dorsum of 15 Sprague-Dawley rats. The wounds were treated with GelMA alone, GelMA+DPSCs, or GelMA+DPSCs+VEGF. Unprotected wounds were used as controls. Animals were euthanized at 1-, 2-, and 4 weeks post-surgery, and the healing wounds were harvested for clinical, histological, and RT-PCR analysis. RESULTS: No signs of clinical inflammation were observed among all groups. Few and sparse mononuclear inflammatory cells were observed in GelMA+DPSCs and GelMA+DPSCs+VEGF groups at 2 weeks, with complete epithelialization of the wounds. At 4 weeks, the epidermis in GelMA+DPSCs and GelMA+DPSCs+VEGF groups was indistinguishable from the empty defect and GelMA groups. The decrease in cellularity and increase in density of collagen fibers were observed over time in both GelMA+DPSCs and GelMA+DPSCs+VEGF groups but were more evident in the GelMA+DPSCs+VEGF group. The GelMA+DPSCs+VEGF group showed a higher expression of the KER 10 gene at all time points compared with the other groups. Expression of Col1 A1 and TGFß-1 were not statistically different over time neither among the groups. CONCLUSION: GelMA scaffolds loaded with DPSCs, and VEGF accelerated the re-epithelialization of skin wounds.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fator A de Crescimento do Endotélio Vascular / Gelatina Limite: Animals / Humans Idioma: En Revista: J Stomatol Oral Maxillofac Surg Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: França
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fator A de Crescimento do Endotélio Vascular / Gelatina Limite: Animals / Humans Idioma: En Revista: J Stomatol Oral Maxillofac Surg Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: França