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Antibacterial activity of silver nanoparticles functionalized with amikacin applied against multidrug-resistant acinetobacter baumannii.
Camargo, Larissa de O; Fontoura, Inglid; Veriato, Thaís S; Raniero, Leandro; Castilho, Maiara L.
Afiliação
  • Camargo LO; Bionanotechnology Laboratory, Research and Development Institute, University of Paraiba Valley, São José dos Campos/SP, Brazil.
  • Fontoura I; Bionanotechnology Laboratory, Research and Development Institute, University of Paraiba Valley, São José dos Campos/SP, Brazil.
  • Veriato TS; Bionanotechnology Laboratory, Research and Development Institute, University of Paraiba Valley, São José dos Campos/SP, Brazil.
  • Raniero L; Nanosensors Laboratory, Research and Development Institute, University of Paraiba Valley, São José dos Campos/SP, Brazil.
  • Castilho ML; Bionanotechnology Laboratory, Research and Development Institute, University of Paraiba Valley, São José dos Campos/SP, Brazil. Electronic address: mcastilho@univap.br.
Am J Infect Control ; 51(8): 871-878, 2023 08.
Article em En | MEDLINE | ID: mdl-36581226
BACKGROUND: Multidrug-resistant bacteria are one of the world's biggest health problems; therefore, improving the spectrum of action of antibiotics could be necessary to reverse this situation. Amikacin and silver salts have well-known antimicrobial properties. However, both drugs lost their effectiveness against some bacteria, such as Acinetobacter baumannii. This work aims to develop a nanodrug from silver nanoparticles (AgNPs) functionalized with Amikacin against multidrug-resistant Acinetobacter baumannii. METHODS: AgNPs were produced using the bottom-up methodology and functionalized with Amikacin modified by the carbodiimide-based chemistry, forming AgNPs@Amikacin. Susceptibility tests were performed using Amikacin-resistant Acinetobacter baumannii strains to assess the bacteriostatic and bactericidal potential of the developed nanodrug. The clinical strains were induced to form a biofilm, and biomass quantification and the metabolic activity were determined. RESULTS: The AgNPs have a hydrodynamic diameter of the particles with a bimodal distribution, with a size of 37.84 nm. The FT-IR spectrum of AgNPs@Amikacin exhibits vibrational modes corresponding to Amikacin, confirming the conjugation to AgNPs. Susceptibility testing demonstrated a minimal inhibitory and bactericidal concentration of < 0.5 µg/mL. The AgNPs@Amikacin reduced the biofilm metabolic activity of Acinetobacter baumannii at rates ≥ 50%, characterized by the minimal biofilm inhibition concentrations. CONCLUSIONS: Results demonstrate a promising development of a new nanodrug with lower concentrations, less toxicity, and greater efficacy against multidrug-resistant Acinetobacter baumannii.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Acinetobacter baumannii / Nanopartículas Metálicas Limite: Humans Idioma: En Revista: Am J Infect Control Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Acinetobacter baumannii / Nanopartículas Metálicas Limite: Humans Idioma: En Revista: Am J Infect Control Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos