Your browser doesn't support javascript.
loading
Iron supplementation does not aggravate impaired glucose tolerance and sugar overload-induced genotoxicity in rats.
Molz, Patrícia; Dallemole, Danieli Rosane; Molz, Walter Augusto; Priebe Steffens, Juliana; Wildner Maluf, Sharbel; Baroni Cruz, Dennis; Rieger, Alexandre; Salvador, Mirian; Prá, Daniel; Rech Franke, Silvia Isabel.
Afiliação
  • Molz P; Laboratory of Experimental Nutrition, University of Santa Cruz do Sul, Santa Cruz do Sul, Brazil.
  • Dallemole DR; Graduate Program in Health Promotion, University of Santa Cruz do Sul, Santa Cruz do Sul, RS, Brazil.
  • Molz WA; Graduate Program in Biosciences, Federal University of Health Sciences of Porto Alegre, Porto Alegre, RS, Brazil.
  • Priebe Steffens J; Laboratory of Histology and Pathology, University of Santa Cruz do Sul, Santa Cruz do Sul, Brazil.
  • Wildner Maluf S; Medicine Course, Department of Biology and Pharmacy, University of Santa Cruz do Sul, Santa Cruz do Sul, Brazil.
  • Baroni Cruz D; Laboratory of Experimental Nutrition, University of Santa Cruz do Sul, Santa Cruz do Sul, Brazil.
  • Rieger A; Laboratory of Cytogenetics and Genome Stability, Graduate Program in Pharmacy and University Hospital, Federal University of Santa Catarina, Florianópolis, SC, Brazil.
  • Salvador M; Medicine Course, Department of Biology and Pharmacy, University of Santa Cruz do Sul, Santa Cruz do Sul, Brazil.
  • Prá D; Graduate Program in Health Promotion, University of Santa Cruz do Sul, Santa Cruz do Sul, RS, Brazil.
  • Rech Franke SI; Laboratory of Oxidative Stress and Antioxidants, University of Caxias do Sul, Caxias do Sul, Brazil.
Mol Cell Biochem ; 478(8): 1719-1725, 2023 Aug.
Article em En | MEDLINE | ID: mdl-36564575
High sugar intake is a major risk factor for metabolic disorders. Genotoxicity is an important factor in diabetes onset, and iron (Fe) may be an aggravating element. However, this relationship is still poorly established. Thus, this study evaluated whether Fe supplementation could aggravate obesity, impaired glucose tolerance, and sugar overload-induced genotoxicity in rats. A total of 24 rats were treated with different diets: standard diet (SD, n = 8), invert sugar overload (320 g/L, HSD, n = 8), or Fe plus invert sugar overload (2.56 mg/L of Fe2+, Fe-HSD, n = 8) for four months. After treatment, the Fe-HSD group showed no excessive weight gain or impaired glucose tolerance. DNA damage in blood, as assessed by comet assay, gradually increased in HSD during treatment (p < 0.001), whereas Fe-HSD showed a nonlinear increase in DNA damage. Moreover, Fe-HSD presented 0.6-fold more DNA damage compared with SD (p = 0.0055) in the 1st month of treatment. At months 2 and 3, results show a ≥ 1.4-fold increase in HSD and Fe-HSD DNA damage, respectively, compared with SD (p < 0.01). At the end of the experiment, only HSD DNA damage differed from SD (1.5-fold more, p = 0.0196). Fe supplementation did not aggravate the invert sugar-induced DNA damage (p > 0.05). In the pancreas, results showed no differences in DNA damage. Mutagenicity, evaluated by micronucleus testing, was not observed regardless of treatment (p = 0.428). Fe supplementation, in the evaluated concentration, did not aggravate weight gain, impaired glucose tolerance, and sugar overload-induced genotoxicity in rats.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Intolerância à Glucose / Ferro Tipo de estudo: Risk_factors_studies Limite: Animals Idioma: En Revista: Mol Cell Biochem Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Intolerância à Glucose / Ferro Tipo de estudo: Risk_factors_studies Limite: Animals Idioma: En Revista: Mol Cell Biochem Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Holanda