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Potential Cytoprotective and Regulatory Effects of Ergothioneine on Gene Expression of Proteins Involved in Erythroid Adaptation Mechanisms and Redox Pathways in K562 Cells.
Bernardo, Victoria Simões; Torres, Flaviene Felix; de Paula, Carla Peres; da Silva, João Pedro Maia de Oliveira; de Almeida, Eduardo Alves; da Cunha, Anderson Ferreira; da Silva, Danilo Grünig Humberto.
Afiliação
  • Bernardo VS; Department of Biology, Universidade Estadual Paulista (UNESP), José do Rio Preto 15054-000, SP, Brazil.
  • Torres FF; Department of Biology, Universidade Estadual Paulista (UNESP), José do Rio Preto 15054-000, SP, Brazil.
  • de Paula CP; Department of Genetics and Evolution, Universidade Federal de São Carlos (UFSCar), São Carlos 13565-905, SP, Brazil.
  • da Silva JPMO; Department of Genetics and Evolution, Universidade Federal de São Carlos (UFSCar), São Carlos 13565-905, SP, Brazil.
  • de Almeida EA; Department of Natural Sciences, Fundação Universidade Regional de Blumenau (FURB), Blumenau 89030-000, SC, Brazil.
  • da Cunha AF; Department of Genetics and Evolution, Universidade Federal de São Carlos (UFSCar), São Carlos 13565-905, SP, Brazil.
  • da Silva DGH; Campus de Três Lagoas, Universidade Federal de Mato Grosso do Sul (CPTL/UFMS), Três Lagoas 79613-000, MS, Brazil.
Genes (Basel) ; 13(12)2022 12 15.
Article em En | MEDLINE | ID: mdl-36553634
This study aimed to establish the importance of ergothioneine (ERT) in the erythroid adaptation mechanisms by appraising the expression levels of redox-related genes associated with the PI3K/AKT/FoxO3 and Nrf2-ARE pathways using K562 cells induced to erythroid differentiation and H2O2-oxidative stress. Cell viability and gene expression were evaluated. Two concentrations of ERT were assessed, 1 nM (C1) and 100 µM (C2), with and without stress induction (100 µM H2O2). Assessments were made in three periods of the cellular differentiation process (D0, D2, and D4). The C1 treatment promoted the induction of FOXO3 (D0 and 2), PSMB5, and 6 expressions (D4); C1 + H2O2 treatment showed the highest levels of NRF2 transcripts, KEAP1 (D0), YWHAQ (D2 and 4), PSMB5 (D2) and PSMB6 (D4); and C2 + H2O2 (D2) an increase in FOXO3 and MST1 expression, with a decrease of YWHAQ and NRF2 was observed. in C2 + H2O2 (D2) an increase in FOXO3 and MST1, with a decrease in YWHAQ and NRF2 was observed All ERT treatments increased gamma-globin expression. Statistical multivariate analyzes highlighted that the Nrf2-ARE pathway presented a greater contribution in the production of PRDX1, SOD1, CAT, and PSBM5 mRNAs, whereas the PI3K/AKT/FoxO3 pathway was associated with the PRDX2 and TRX transcripts. In conclusion, ERT presented a cytoprotective action through Nrf2 and FoxO3, with the latter seeming to contribute to erythroid proliferation/differentiation.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ergotioneína Limite: Humans Idioma: En Revista: Genes (Basel) Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ergotioneína Limite: Humans Idioma: En Revista: Genes (Basel) Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Suíça