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Geniposide and asperuloside alter the COX-2 and GluN2B receptor expression after pilocarpine-induced seizures in mice.
Uczay, Mariana; Pflüger, Pricila; Picada, Jaqueline Nascimento; de Oliveira, João Denis Medeiros; da SilvaTorres, Iraci Lucena; Medeiros, Helouise Richardt; Vendruscolo, Maria Helena; von Poser, Gilsane; Pereira, Patrícia.
Afiliação
  • Uczay M; Laboratory of Neuropharmacology and Preclinical Toxicology, Institute of Basic Health Sciences, Federal University of Rio Grande Do Sul, Porto Alegre, 90050-170, Brazil.
  • Pflüger P; Laboratory of Neuropharmacology and Preclinical Toxicology, Institute of Basic Health Sciences, Federal University of Rio Grande Do Sul, Porto Alegre, 90050-170, Brazil.
  • Picada JN; Laboratory of Genetic Toxicology, Lutheran University of Brazil, 92425-900, Canoas São José, Brazil.
  • de Oliveira JDM; Laboratory of Genetic Toxicology, Lutheran University of Brazil, 92425-900, Canoas São José, Brazil.
  • da SilvaTorres IL; Laboratory of Pain Pharmacology and Neuromodulation, Federal University of Rio Grande Do Sul, Porto Alegre, 90050-170, Brazil.
  • Medeiros HR; Laboratory of Pain Pharmacology and Neuromodulation, Federal University of Rio Grande Do Sul, Porto Alegre, 90050-170, Brazil.
  • Vendruscolo MH; Laboratory of Pharmacognosy, College of Pharmacy, Federal University of Rio Grande Do Sul (UFRGS), Porto Alegre, 90050-170, Brazil.
  • von Poser G; Laboratory of Pharmacognosy, College of Pharmacy, Federal University of Rio Grande Do Sul (UFRGS), Porto Alegre, 90050-170, Brazil.
  • Pereira P; Laboratory of Neuropharmacology and Preclinical Toxicology, Institute of Basic Health Sciences, Federal University of Rio Grande Do Sul, Porto Alegre, 90050-170, Brazil. patriciapereira@ufrgs.br.
Naunyn Schmiedebergs Arch Pharmacol ; 396(5): 951-962, 2023 05.
Article em En | MEDLINE | ID: mdl-36536207
Asperuloside (ASP) and geniposide (GP) are iridoids that have shown various biological properties, such as reduction of inflammation, oxidative stress, and neuroprotection. The aim of this study was to investigate the mechanism of action of ASP and GP through the experimental model of pilocarpine-induced seizures. Mice were treated daily with saline, valproic acid (VPA), GP (5, 25, or 50 mg/kg), or ASP (20 or 40 mg/kg) for 8 days. Pilocarpine (PILO) treatment was administered after the last day of treatment, and the epileptic behavior was recorded for 1 h and analyzed by an adapted scale. Afterward, the hippocampus and blood samples were collected for western blot analyses, ELISA and comet assay, and bone marrow to the micronucleus test. We evaluated the expression of the inflammatory marker cyclooxygenase-2 (COX-2), GluN2B, a subunit of the NMDA receptor, pGluR1, an AMPA receptor, and the enzyme GAD-1 by western blot and the cytokine TNF-α by ELISA. The treatments with GP and ASP were capable to decrease the latency to the first seizure, although they did not change the latency to status epilepticus (SE). ASP demonstrated a genotoxic potential analyzed by comet assay; however, the micronuclei frequency was not increased in the bone marrow. The GP and ASP treatments were capable to reduce COX-2 and GluN2B receptor expression after PILO exposure. This study suggests that GP and ASP have a protective effect on PILO-induced seizures, decreasing GluN2B receptor and COX-2 expression.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pilocarpina / Receptores de N-Metil-D-Aspartato Limite: Animals Idioma: En Revista: Naunyn Schmiedebergs Arch Pharmacol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Pilocarpina / Receptores de N-Metil-D-Aspartato Limite: Animals Idioma: En Revista: Naunyn Schmiedebergs Arch Pharmacol Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha