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The preventive use of resveratrol increases its antioxidant effect by SIRT1 and subclinical anti-inflammatory action in Neuro-2A cells.
Lara, Raquel Cunha; Franco, Filipe Nogueira; de Araújo, Glaucy Rodrigues; Silva, Barbara Nellita Moura; Chaves, Miriam Martins.
Afiliação
  • Lara RC; Biochemistry Laboratory of Aging and Correlated Diseases, Department of Biochemistry and Immunology, Biological Sciences Institute, Federal University of Minas Gerais, Av. Antônio Carlos 6627, CP 486, Belo Horizonte, MG, 30161-970, Brazil.
  • Franco FN; Biochemistry Laboratory of Aging and Correlated Diseases, Department of Biochemistry and Immunology, Biological Sciences Institute, Federal University of Minas Gerais, Av. Antônio Carlos 6627, CP 486, Belo Horizonte, MG, 30161-970, Brazil.
  • de Araújo GR; Biochemistry Laboratory of Aging and Correlated Diseases, Department of Biochemistry and Immunology, Biological Sciences Institute, Federal University of Minas Gerais, Av. Antônio Carlos 6627, CP 486, Belo Horizonte, MG, 30161-970, Brazil.
  • Silva BNM; Biochemistry Laboratory of Aging and Correlated Diseases, Department of Biochemistry and Immunology, Biological Sciences Institute, Federal University of Minas Gerais, Av. Antônio Carlos 6627, CP 486, Belo Horizonte, MG, 30161-970, Brazil.
  • Chaves MM; Biochemistry Laboratory of Aging and Correlated Diseases, Department of Biochemistry and Immunology, Biological Sciences Institute, Federal University of Minas Gerais, Av. Antônio Carlos 6627, CP 486, Belo Horizonte, MG, 30161-970, Brazil. miriamschultz62@gmail.com.
In Vitro Cell Dev Biol Anim ; 58(10): 979-986, 2022 Dec.
Article em En | MEDLINE | ID: mdl-36481976
Currently, the important role of oxidative stress in the aging process and in neurodegenerative diseases has been highlighted, suggesting the beneficial effect of antioxidants as adjuvant therapy. Resveratrol (RSV) is a polyphenolic compound used in the clinic and has been shown as an antioxidant and anti-inflammatory. Therefore, the objective was to verify neuroprotective and modulating effects of RSV on N2-A cells, pre or post inserted into an oxidative stress environment. For this, two treatment conditions were established: pre-stimulus and post-stimulus. The analysis of AMPK and SIRT1 cell signaling pathways was performed through the chemiluminescence assay using the dorsomorphin and EX527 inhibitors, respectively. The inflammatory profile was also evaluated in these neural cells, through the levels of IL-6, TNF, and IL-10. We observed that RSV in N2-A cells has anti-inflammatory effect and antioxidant property and it mechanism is dependent on the SIRT1 signaling pathway. RSV effects occurs most markedly when cells have been pre-stimulated before inducing an oxidative stress environment. These results are important for conducting more adequate protocols in the medical and nutritional clinic.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sirtuína 1 / Antioxidantes Tipo de estudo: Guideline Limite: Animals Idioma: En Revista: In Vitro Cell Dev Biol Anim Assunto da revista: BIOLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha
Texto completo
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sirtuína 1 / Antioxidantes Tipo de estudo: Guideline Limite: Animals Idioma: En Revista: In Vitro Cell Dev Biol Anim Assunto da revista: BIOLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Alemanha