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Electrochemical biosensor for quantitative determination of fentanyl based on immobilized cytochrome c on multi-walled carbon nanotubes modified screen-printed carbon electrodes.
González-Hernández, Jerson; Moya-Alvarado, Guillermo; Alvarado-Gámez, Ana Lorena; Urcuyo, Roberto; Barquero-Quirós, Miriam; Arcos-Martínez, María Julia.
Afiliação
  • González-Hernández J; Centro de Investigación en Electroquímica Y Energía Química (CELEQ), Universidad de Costa Rica, San José, 11501-2060, Costa Rica. jerson.gonzalez@ucr.ac.cr.
  • Moya-Alvarado G; Escuela de Química, Universidad de Costa Rica, San José, 11501-2060, Costa Rica. jerson.gonzalez@ucr.ac.cr.
  • Alvarado-Gámez AL; Escuela de Química, Universidad de Costa Rica, San José, 11501-2060, Costa Rica.
  • Urcuyo R; Centro de Investigación en Electroquímica Y Energía Química (CELEQ), Universidad de Costa Rica, San José, 11501-2060, Costa Rica.
  • Barquero-Quirós M; Centro de Investigación en Electroquímica Y Energía Química (CELEQ), Universidad de Costa Rica, San José, 11501-2060, Costa Rica.
  • Arcos-Martínez MJ; Escuela de Química, Universidad de Costa Rica, San José, 11501-2060, Costa Rica.
Mikrochim Acta ; 189(12): 483, 2022 11 30.
Article em En | MEDLINE | ID: mdl-36446842
Fentanyl is a powerful synthetic opioid used to treat severe pain. New administration routes toward its illegal consumption for recreational purposes pose a growing threat to public health, either due to misuse or abuse of this substance. As a result, the rapid qualitative and quantitative determination of fentanyl in biofluids is of great interest. A novel enzymatic biosensor based on adsorptive-stripping cyclic voltammetry is proposed as a cost-effective, reliable, and efficient device for fentanyl determination in urine samples. Disposable screen-printed carbon electrodes modified with multi-walled carbon nanotubes and cytochrome c were used to develop the testing platform. The electrochemical behavior of fentanyl exhibited a well-defined anodic wave around 0.66 V vs. pseudo reference electrode. The experimental conditions were optimized to obtain the best analytical response, and linear regression analysis of increasing concentration standards was applied to estimate the performance parameters. The results suggest a simple method with a wide linearity range, high sensitivity, low limits of detection (0.086 µg/mL) and quantification, and satisfactory precision (2.9% RSD). The feasibility and applicability of the voltammetric approach were assessed by fentanyl-spiked urine samples by standard additions calibration curves in two levels of enrichment with an accuracy of 92% and 100%.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Nanotubos de Carbono Tipo de estudo: Qualitative_research Idioma: En Revista: Mikrochim Acta Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Costa Rica País de publicação: Áustria

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Nanotubos de Carbono Tipo de estudo: Qualitative_research Idioma: En Revista: Mikrochim Acta Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Costa Rica País de publicação: Áustria