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A Free Fatty Acid Synthetic Agonist Accelerates Wound Healing and Improves Scar Quality in Mice.
Prado, Thais P; Jara, Carlos P; Dias Bóbbo, Vanessa C; Carraro, Rodrigo S; Sidarta-Oliveira, Davi; de Mendonça, Guilherme R A; Velloso, Licio A; Araújo, Eliana P.
Afiliação
  • Prado TP; Nursing School, Laboratory of Cell Signaling Obesity and Comorbidities Center, OCRC, University of Campinas, Campinas, Brazil.
  • Jara CP; Faculty of Medical Sciences, Laboratory of Cell Signaling, Obesity and Comorbidities Center - OCRC, University of Campinas, Campinas, Brazil.
  • Dias Bóbbo VC; Nursing School, Laboratory of Cell Signaling Obesity and Comorbidities Center, OCRC, University of Campinas, Campinas, Brazil.
  • Carraro RS; Faculty of Medical Sciences, Laboratory of Cell Signaling, Obesity and Comorbidities Center - OCRC, University of Campinas, Campinas, Brazil.
  • Sidarta-Oliveira D; Nursing School, Laboratory of Cell Signaling Obesity and Comorbidities Center, OCRC, University of Campinas, Campinas, Brazil.
  • de Mendonça GRA; Faculty of Medical Sciences, Laboratory of Cell Signaling, Obesity and Comorbidities Center - OCRC, University of Campinas, Campinas, Brazil.
  • Velloso LA; Faculty of Medical Sciences, Laboratory of Cell Signaling, Obesity and Comorbidities Center - OCRC, University of Campinas, Campinas, Brazil.
  • Araújo EP; Faculty of Medical Sciences, University of Campinas, Campinas, Brazil.
Biol Res Nurs ; 25(3): 353-366, 2023 07.
Article em En | MEDLINE | ID: mdl-36444640
BACKGROUND: Impaired wound healing is a health problem around the world, and the search for a novel product to repair wounded skin is a major topic in the field. GW9508 is a synthetic molecule described as a selective agonist of free fatty acid receptors (FFARs) 1 and 4, and there is evidence of its anti-inflammatory effects on several organs of the body. PURPOSE: Here, we aimed to evaluate the effects of topical GW9508 on wound healing in mice. RESEARCH DESIGN: First, we used bioinformatic methods to determine the expression of FFAR1 and FFAR4 mRNA in the skin from a human cell atlas assembled with single-cell transcriptomes. Next, we employed 6-week-old C57BL6J mice with 2 wounds inflicted in the back. The mice were randomly divided into 2 groups, a control group, which received topical vehicle, and a treatment group, which received GW9508, for 12 days. The wound was monitored by photographic documentation every 2 days, and samples were collected at day 6 and 12 post injury for RT-PCR, western blot and histology analyses. RESULTS: FFAR1 and FFAR4 mRNA are expressed in skin cells in similar amounts to those in other tissues. Topical GW9508 accelerated wound healing and decreased gene expression of IL-10 and metalloproteinase 9 on days 6 and 12 post injury. It increased the quantity of Collagen I and improved the organization of collagen fibres. Conclusions: Our results show that GW9508 could be an attractive drug treatment for wounded skin. Future studies need to be performed to assess the impact of GW9508 in chronic wound models.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cicatrização / Cicatriz / Metilaminas Limite: Animals Idioma: En Revista: Biol Res Nurs Assunto da revista: ENFERMAGEM / MEDICINA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Cicatrização / Cicatriz / Metilaminas Limite: Animals Idioma: En Revista: Biol Res Nurs Assunto da revista: ENFERMAGEM / MEDICINA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Estados Unidos