Your browser doesn't support javascript.
loading
Prevalence of three Mycoplasma sp. by multiplex PCR in cattle with and without respiratory disease in central Mexico.
Maya-Rodríguez, L M; Carrillo-Casas, E M; Rojas-Trejo, V; Trigo-Tavera, F; Miranda-Morales, R E.
Afiliação
  • Maya-Rodríguez LM; Laboratorio de Mycoplasmas, Facultad de Medicina Veterinaria y Zootecnia UNAM, Departamento de Microbiología e Inmunología, Ciudad Universitaria, 04519, CDMX, CP, Mexico.
  • Carrillo-Casas EM; Hospital General "Dr. Manuel Gea González", Depto. de Biología Molecular e Histocompatibilidad, Dirección de Investigación, Calz. de Tlalpan 4800, Secc XVI, 14080, Tlalpan CDMX, CP, Mexico.
  • Rojas-Trejo V; Laboratorio de Mycoplasmas, Facultad de Medicina Veterinaria y Zootecnia UNAM, Departamento de Microbiología e Inmunología, Ciudad Universitaria, 04519, CDMX, CP, Mexico.
  • Trigo-Tavera F; Facultad de Medicina Veterinaria y Zootecnia UNAM, Departamento de Patología, Ciudad Universitaria, 04519, CDMX, CP, Mexico.
  • Miranda-Morales RE; Laboratorio de Mycoplasmas, Facultad de Medicina Veterinaria y Zootecnia UNAM, Departamento de Microbiología e Inmunología, Ciudad Universitaria, 04519, CDMX, CP, Mexico. roelmimo@yahoo.com.mx.
Trop Anim Health Prod ; 54(6): 394, 2022 Nov 22.
Article em En | MEDLINE | ID: mdl-36417039
This study aimed to identify Mycoplasma bovis, Myc. dispar, and Myc. bovirhinis, which are involved in bovine respiratory disease through a multiplex PCR as an alternative to culture's features that hamper Mycoplasma isolation. Nasal swabs were taken from 335 cattle with and without respiratory disease background (RDB) from dairy herds in the central region of Mexico. Each sample was divided in two; the first part was processed for the direct DNA extraction of the nasal swab and the second for Mycoplasma isolation, culture, and then the multiplex PCR was performed. In the nasal swabs, Myc. bovis was identified in 21.1%; Myc. dispar, in 11.8%; and Myc. bovirhinis, in 10.8% in cattle with RDB. Isolates were identified as Myc. bovis, 20.1%; Myc. dispar, 11.8%; and Myc. bovirhinis, 6.1%. There is a strong correlation between the presence of Mycoplasma identified by PCR and the clinical history of the disease (ρ < 0.0000). In animals without RDB, Myc. bovirhinis was the only species detected in 6.1% of the samples processed directly for multiplex PCR, and in 2% of the isolates. There is an excellent correlation (kappa 0.803) between the isolation and the 16S PCR and a high correlation (kappa 0.75) between the isolation and the multiplex PCR. Therefore, we conclude that the PCR multiplex test is highly sensitive and may be used for the diagnosis and surveillance of the three species in biological samples and mycoplasma isolates.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transtornos Respiratórios / Doenças Respiratórias / Doenças dos Bovinos / Mycoplasma bovis Tipo de estudo: Diagnostic_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Animals País/Região como assunto: Mexico Idioma: En Revista: Trop Anim Health Prod Ano de publicação: 2022 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Transtornos Respiratórios / Doenças Respiratórias / Doenças dos Bovinos / Mycoplasma bovis Tipo de estudo: Diagnostic_studies / Prevalence_studies / Prognostic_studies / Risk_factors_studies Limite: Animals País/Região como assunto: Mexico Idioma: En Revista: Trop Anim Health Prod Ano de publicação: 2022 Tipo de documento: Article País de afiliação: México País de publicação: Estados Unidos