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Chromatographic Techniques and Pharmacological Analysis as a Quality Control Strategy for Serjania triquetra a Traditional Medicinal Plant.
Aguilar-Guadarrama, A Berenice; Yáñez-Ibarra, Guadalupe; Cancino-Marentes, Martha Edith; González-Ibarra, Paola; Ortiz-Andrade, Rolffy; Sánchez-Recillas, Amanda; Rodríguez-Carpena, Javier-German; Aguirre-Vidal, Yoshajandith; Medina-Diaz, Irma-Martha; Ávila-Villarreal, Gabriela.
Afiliação
  • Aguilar-Guadarrama AB; Centro de Investigaciones Químicas, IICBA, Universidad Autónoma del Estado de Morelos, Av. Universidad 1001, Col. Chamilpa, Cuernavaca 62209, Mexico.
  • Yáñez-Ibarra G; Centro Nayarita de Innovación y Transferencia de Tecnología A. C. "Unidad Especializada en I+D+i en Calidad de Alimentos y Productos Naturales", Universidad Autónoma de Nayarit, Tepic 63000, Mexico.
  • Cancino-Marentes ME; Unidad Académica de Medicina Humana, Universidad Autónoma de Nayarit, Tepic 63000, Mexico.
  • González-Ibarra P; Unidad Académica de Salud Integral, Universidad Autónoma de Nayarit, Tepic 63000, Mexico.
  • Ortiz-Andrade R; Laboratorio de Farmacología, Facultad de Química, Universidad Autónoma de Yucatán, Mérida 97069, Mexico.
  • Sánchez-Recillas A; Laboratorio de Farmacología, Facultad de Química, Universidad Autónoma de Yucatán, Mérida 97069, Mexico.
  • Rodríguez-Carpena JG; Centro Nayarita de Innovación y Transferencia de Tecnología A. C. "Unidad Especializada en I+D+i en Calidad de Alimentos y Productos Naturales", Universidad Autónoma de Nayarit, Tepic 63000, Mexico.
  • Aguirre-Vidal Y; Red de Estudios Moleculares Avanzados, Campus III, Instituto de Ecología A.C. (INECOL), Xalapa 91073, Mexico.
  • Medina-Diaz IM; Laboratorio de Contaminación y Toxicología Ambiental, Secretaría de Investigación y Posgrado, Universidad Autónoma de Nayarit, Tepict 63000, Mexico.
  • Ávila-Villarreal G; Centro Nayarita de Innovación y Transferencia de Tecnología A. C. "Unidad Especializada en I+D+i en Calidad de Alimentos y Productos Naturales", Universidad Autónoma de Nayarit, Tepic 63000, Mexico.
Pharmaceuticals (Basel) ; 15(10)2022 Oct 20.
Article em En | MEDLINE | ID: mdl-36297401
Serjania triquetra is a medicinal plant widely used in traditional medicine for the treatment of urinary tract diseases, renal affections, and its complications. The population can buy this plant in folk markets as a raw material mixed with several herbal remedies or as a health supplement. On the market, two commercial presentations were found for the vegetal material; one had a bulk appearance and the other was marketed wrapped in cellophane bags (HESt-2, HESt-3). Nevertheless, the plant has not been exhaustively investigated and quality control techniques have not been developed. This research aimed to realize a phytochemical study using an authentic, freshly collected sample as a reference for S. triquetra (HESt-1), using the compounds identified. A method for the determination of preliminary chromatographic fingerprinting was developed. Additionally, the vasorelaxant effect from three samples was evaluated with ex vivo rat models. Thus, three hydroalcoholic extracts (HESt-1, HESt-2, and HESt-3) were prepared by maceration. A total of nine compounds were fully identified from HESt-1 after the extract was subjected to open-column chromatography. Seven metabolites were detected by gas chromatography, while ursolic acid (UA) and allantoin were isolated and identified using UPLC-MS and NMR, respectively. Three extracts were analyzed for their chromatographic fingerprint by UPLC-MS. Biological activity was explored by ex vivo rat aorta ring model to evaluate vasorelaxant activity. All extracts showed a vasorelaxant effect in a concentration-dependent and endothelium-dependent manner. S. triquetra vascular activity may be attributed to UA and allantoin compounds previously described in the literature for this activity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Pharmaceuticals (Basel) Ano de publicação: 2022 Tipo de documento: Article País de afiliação: México País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Pharmaceuticals (Basel) Ano de publicação: 2022 Tipo de documento: Article País de afiliação: México País de publicação: Suíça