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Anaerobiosis favors biosynthesis of single and multi-element nanostructures.
Ríos-Silva, Mirtha; Pérez, Myriam; Luraschi, Roberto; Vargas, Esteban; Silva-Andrade, Claudia; Valdés, Jorge; Sandoval, Juan Marcelo; Vásquez, Claudio; Arenas, Felipe.
Afiliação
  • Ríos-Silva M; Laboratorio de Microbiología Molecular, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile.
  • Pérez M; Research Center on the Intersection in Plasma Physics, Matter and Complexity, P2mc, Comisión Chilena de Energía Nuclear, Santiago, Chile.
  • Luraschi R; Laboratorio de Microbiología Molecular, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile.
  • Vargas E; Laboratorio de Microbiología Molecular, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile.
  • Silva-Andrade C; Center for the Development of Nanoscience and Nanotechnology (CEDENNA), Santiago, Chile.
  • Valdés J; Centro de Genómica y Bioinformática, Universidad Mayor, Santiago, Chile.
  • Sandoval JM; Centro de Genómica y Bioinformática, Universidad Mayor, Santiago, Chile.
  • Vásquez C; Facultad de Ciencias, Universidad Arturo Prat, Iquique, Chile.
  • Arenas F; Laboratorio de Microbiología Molecular, Departamento de Biología, Facultad de Química y Biología, Universidad de Santiago de Chile, Santiago, Chile.
PLoS One ; 17(10): e0273392, 2022.
Article em En | MEDLINE | ID: mdl-36206251
Herein we report the use of an environmental multimetal(loid)-resistant strain, MF05, to biosynthesize single- or multi-element nanostructures under anaerobic conditions. Inorganic nanostructure synthesis typically requires methodologies and conditions that are harsh and environmentally hazardous. Thus, green/eco-friendly procedures are desirable, where the use of microorganisms and their extracts as bionanofactories is a reliable strategy. First, MF05 was entirely sequenced and identified as an Escherichia coli-related strain with some genetic differences from the traditional BW25113. Secondly, we compared the CdS nanostructure biosynthesis by whole-cell in a design defined minimal culture medium containing sulfite as the only sulfur source to obtain sulfide reduction from a low-cost chalcogen reactant. Under anaerobic conditions, this process was greatly favored, and irregular CdS (ex. 370 nm; em. 520-530 nm) was obtained. When other chalcogenites were tested (selenite and tellurite), only spherical Se0 and elongated Te0 nanostructures were observed by TEM and analyzed by SEM-EDX. In addition, enzymatic-mediated chalcogenite (sulfite, selenite, and tellurite) reduction was assessed by using MF05 crude extracts in anaerobiosis; similar results for nanostructures were obtained; however Se0 and Te0 formation were more regular in shape and cleaner (with less background). Finally, the in vitro nanostructure biosynthesis was assessed with salts of Ag, Au, Cd, and Li alone or in combination with chalcogenites. Several single or binary nanostructures were detected. Our results showed that MF05 is a versatile anaerobic bionanofactory for different types of inorganic NS. synthesis.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sais / Nanoestruturas Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Sais / Nanoestruturas Idioma: En Revista: PLoS One Assunto da revista: CIENCIA / MEDICINA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Chile País de publicação: Estados Unidos