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Melatonin protects against ovarian damage by inhibiting autophagy in granulosa cells in rats.
Liu, Yan; Zhu, Xiaohe; Wu, Chunli; Lang, Yan; Zhao, Wenjie; Li, Yanmin.
Afiliação
  • Liu Y; Department of Gynecology, Weifang People's Hospital, Weifang, Shandong, China.
  • Zhu X; Department of Obstetrics, Weifang People's Hospital, Weifang, Shandong, China.
  • Wu C; Department of Obstetrics, Weifang People's Hospital, Weifang, Shandong, China.
  • Lang Y; Department of Obstetrics, Weifang People's Hospital, Weifang, Shandong, China.
  • Zhao W; Department of Reproductive Medicine, Weifang People's Hospital, Weifang, Shandong, China.
  • Li Y; Department of Reproductive Medicine, Weifang People's Hospital, Weifang, Shandong, China. Electronic address: yanminli66@163.com.
Clinics (Sao Paulo) ; 77: 100119, 2022.
Article em En | MEDLINE | ID: mdl-36194922
OBJECTIVES: This study sought to further verify the protective mechanism of Melatonin (MT) against ovarian damage through animal model experiments and to lay a theoretical and experimental foundation for exploring new approaches for ovarian damage treatment. METHOD: The wet weight and ovarian index of rat ovaries were weighted, and the morphology of ovarian tissues and the number of follicles in the pathological sections of collected ovarian tissues were recorded. And the serum sex hormone levels, the key proteins of the autophagy pathway (PI3K, AKT, mTOR, LC3II, LC3I, and Agt5) in rat ovarian tissues, as well as the viability and mortality of ovarian granulosa cells in each group were measured by ELISA, western blotting, CCK8 kit and LDH kit, respectively. RESULTS: The results showed that MT increased ovarian weight and improved the ovarian index in ovarian damage rats. Also, MT could improve autophagy-induced ovarian tissue injury, increase the number of primordial follicles, primary follicles, and sinus follicles, and decrease the number of atretic follicles. Furthermore, MT upregulated serum AMH, INH-B, and E2 levels downregulated serum FSH and LH levels in ovarian damage rats and activated the PI3K/AKT/mTOR signaling pathway. Besides, MT inhibited autophagic apoptosis of ovarian granulosa cells and repressed the expression of key proteins in the autophagic pathway and reduced the expression levels of Agt5 and LC3II/I. CONCLUSIONS: MT inhibits granulosa cell autophagy by activating the PI3K/Akt/mTOR signaling pathway, thereby exerting a protective effect against ovarian damage.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ovário / Melatonina Limite: Animals Idioma: En Revista: Clinics (Sao Paulo) Assunto da revista: MEDICINA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Ovário / Melatonina Limite: Animals Idioma: En Revista: Clinics (Sao Paulo) Assunto da revista: MEDICINA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China País de publicação: Estados Unidos