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Clinical Value of Serial Quantitative Analysis of Cytomegalovirus DNA in Blood and Saliva Over the First 24 Months of Life in Congenital Infection: The French Cymepedia Cohort.
Fourgeaud, Jacques; Magny, Jean-François; Couderc, Sophie; Garcia, Patricia; Maillotte, Anne-Marie; Benard, Melinda; Pinquier, Didier; Minodier, Philippe; Astruc, Dominique; Patural, Hugues; Ugolin, Melissa; Parat, Sophie; Guillois, Bernard; Garenne, Armelle; Guilleminot, Tiffany; Parodi, Marine; Bussières, Laurence; Ville, Yves; Leruez-Ville, Marianne.
Afiliação
  • Fourgeaud J; Research Unit 73-28, Université Paris Cité, Paris, France; Virology Laboratory, Reference Laboratory for Cytomegalovirus Infections, AP-HP, Hôpital Necker Enfants Malades, Paris, France. Electronic address: jacques.fourgeaud@aphp.fr.
  • Magny JF; Research Unit 73-28, Université Paris Cité, Paris, France; Neonatal Intensive Care Unit, AP-HP, Hôpital Necker Enfants Malades, Paris, France.
  • Couderc S; Maternity, Hospital Intercommunal Poissy-Saint Germain, Poissy, France.
  • Garcia P; Neonatology and Intensive Care Department, AP-HM, Hospital La Conception, Marseille, France.
  • Maillotte AM; Neonatal Intensive Care Unit, CHU Nice, Hospital L'Archet, Nice, France.
  • Benard M; Department of Neonatalogy, Toulouse University Hospital, Toulouse, France.
  • Pinquier D; Department of Neonatology, Rouen University Hospital, Rouen, France.
  • Minodier P; Emergency Care Department, AP-HM, Hospital Nord, Marseille, France.
  • Astruc D; Department of Neonatology, Strasbourg University Hospital, Strasbourg, France.
  • Patural H; Neonatal Intensive Care Unit, University Hospital, Saint-Etienne, France.
  • Ugolin M; Pediatric Department, Neonatology, CHU Rennes and CIC1414, Rennes, France.
  • Parat S; Maternity, AP-HP, Hospital Cochin, Paris, France.
  • Guillois B; Department of Neonatalogy, CHU de Caen, Caen, France; Medical School, Université Caen Normandie, Caen, France.
  • Garenne A; Neonatal and Pediatric Intensive Care Unit, CHRU Brest, Brest, France.
  • Guilleminot T; Research Unit 73-28, Université Paris Cité, Paris, France; Virology Laboratory, Reference Laboratory for Cytomegalovirus Infections, AP-HP, Hôpital Necker Enfants Malades, Paris, France.
  • Parodi M; Otology Department, AP-HP, Hôpital Necker Enfants Malades, Paris, France.
  • Bussières L; Research Unit 73-28, Université Paris Cité, Paris, France; Clinical Research Unit, AP-HP, Hôpital Necker Enfants Malades, Paris, France.
  • Ville Y; Research Unit 73-28, Université Paris Cité, Paris, France; Maternity, AP-HP, Hôpital Necker Enfants Malades, Paris, France.
  • Leruez-Ville M; Research Unit 73-28, Université Paris Cité, Paris, France; Virology Laboratory, Reference Laboratory for Cytomegalovirus Infections, AP-HP, Hôpital Necker Enfants Malades, Paris, France.
J Pediatr ; 253: 197-204.e5, 2023 02.
Article em En | MEDLINE | ID: mdl-36181870
OBJECTIVE: To evaluate cytomegalovirus (CMV) viral load dynamics in blood and saliva during the first 2 years of life in symptomatic and asymptomatic infected infants and to identify whether these kinetics could have practical clinical implications. STUDY DESIGN: The Cymepedia cohort prospectively included 256 congenitally infected neonates followed for 2 years. Whole blood and saliva were collected at inclusion and months 4 and 12, and saliva at months 18 and 24. Real-time CMV polymerase chain reaction (PCR) was performed, results expressed as log10 IU/mL in blood and in copies per milliliter in saliva. RESULTS: Viral load in saliva progressively decreased from 7.5 log10 at birth to 3.3 log10 at month 24. CMV PCR in saliva was positive in 100% and 96% of infants at 6 and 12 months, respectively. In the first month of life, neonatal saliva viral load of less than 5 log10 was related to a late CMV transplacental passage. Detection in blood was positive in 92% of neonates (147/159) in the first month of life. No viral load threshold values in blood or saliva could be associated with a high risk of sequelae. Neonatal blood viral load of less than 3 log10 IU/mL had a 100% negative predictive value for long-term sequelae. CONCLUSIONS: Viral loads in blood and saliva by CMV PCR testing in congenital infection fall over the first 24 months. In this study of infants affected mainly after primary maternal infection during pregnancy, all salivary samples were positive in the first 6 months of life and sequelae were not seen in infants with neonatal blood viral load of less than 3 log10 IU/mL.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Citomegalovirus / Doenças do Recém-Nascido Tipo de estudo: Prognostic_studies / Risk_factors_studies Aspecto: Patient_preference Limite: Female / Humans / Infant / Newborn / Pregnancy Idioma: En Revista: J Pediatr Ano de publicação: 2023 Tipo de documento: Article País de publicação: Estados Unidos
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Infecções por Citomegalovirus / Doenças do Recém-Nascido Tipo de estudo: Prognostic_studies / Risk_factors_studies Aspecto: Patient_preference Limite: Female / Humans / Infant / Newborn / Pregnancy Idioma: En Revista: J Pediatr Ano de publicação: 2023 Tipo de documento: Article País de publicação: Estados Unidos