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Improving the sensitivity for hydrogen peroxide determination with active V2O5 nanocubes incorporated on mesoporous TiO2.
Manoj, Devaraj; Rajendran, Saravanan; Gracia, F; Ansar, Sabah; Santhamoorthy, Madhappan; Soto-Moscoso, Matias; Gracia-Pinilla, M A.
Afiliação
  • Manoj D; Departamento de Ingeniería Mecánica, Facultad de Ingeniería, Universidad de Tarapacá, Avda. General Velásquez, 1775, Arica, Chile.
  • Rajendran S; Departamento de Ingeniería Mecánica, Facultad de Ingeniería, Universidad de Tarapacá, Avda. General Velásquez, 1775, Arica, Chile; Saveetha School of Engineering, Saveetha Institute of Medical and Technical Science, Chennai, 60210, India. Electronic address: saravanan3.raj@gmail.com.
  • Gracia F; Department of Chemical Engineering, Biotechnology and Materials, University of Chile, Beauchef 851, 6th Floor, Santiago, Chile.
  • Ansar S; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Saud University, P.O. Box 10219, Riyadh, 11433, Saudi Arabia.
  • Santhamoorthy M; School of Chemical Engineering, Yeungnam University, Gyeongsan, 38544, Republic of Korea.
  • Soto-Moscoso M; Universidad Autónoma de Chile, Chile.
  • Gracia-Pinilla MA; Universidad Autónoma de Nuevo León, Facultad de Ciencias Físico-Matemáticas, Av. Universidad, Cd. Universitaria, San Nicolás de Los Garza, NL, Mexico; Universidad Autónoma de Nuevo León, Centro de Investigación en Innovación y Desarrollo en Ingeniería y Tecnología, PIIT, Apodaca, N.L., Mexico.
Environ Res ; 215(Pt 3): 114427, 2022 12.
Article em En | MEDLINE | ID: mdl-36179884
The capacity to generate a constant signal response from an enzyme on an electrode surface has been a fascinating topic of research from the past three decades. To nourish the enzymatic activity during electrochemical reactions, the immobilization of dual enzymes on the electrode surface could prevent the enzymatic loss without denaturation and thus long-term stability can be achieved. For effective immobilization of dual enzymes, mesoporous materials are the ideal choice because of its numerous advantages such as 1. The presence of porous structure facilitates high loading of enzymes 2. The formation of protective environment can withstand the enzymatic activity even at acidic or basic pH values and even at elevated temperatures. Herein, we develop bienzymatic immobilization of horseradish peroxidase (HRP) and cholesterol oxidase (ChOx) on mesoporous V2O5-TiO2 based binary nanocomposite for effective sensing of hydrogen peroxide (H2O2) in presence of redox mediator hydroquinone (HQ). The utilization of redox mediator in second-generation biosensing of H2O2 can eliminate the interference species and reduces the operating potential with higher current density for electrochemical reduction reaction. Using this mediator transfer process approach at HRP/ChOx/V2O5-TiO2 modified GC, the H2O2 can be determined at operating potential (-0.2 V) with good linear range (0.05-3.5 mM) higher sensitivity (1040 µAµM-1 cm-2) and lower detection limit of about 20 µM can be attained, which is due to higher mediation of electrons were transferred to the enzyme cofactors. These interesting characteristics could be due to mesoporous structure of V2O5-TiO2 can induce large immobilization and facilitate higher interaction with enzymes for wide range of biosensing applications.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Peróxido de Hidrogênio Tipo de estudo: Diagnostic_studies Idioma: En Revista: Environ Res Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Chile País de publicação: Holanda
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Técnicas Biossensoriais / Peróxido de Hidrogênio Tipo de estudo: Diagnostic_studies Idioma: En Revista: Environ Res Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Chile País de publicação: Holanda