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Antioxidant Potential of the Sweet Whey-Based Beverage Colada after the Digestive Process and Relationships with the Lipid and Protein Fractions.
García-Casas, Victoria E; Seiquer, Isabel; Pardo, Zaira; Haro, Ana; Recio, Isidra; Olías, Raquel.
Afiliação
  • García-Casas VE; Facultad de Ingeniería Química, Universidad de Guayaquil, Ciudadela Universitaria, Avenida Delta S/N y Avenida Kennedy, Guayaquil 090514, Ecuador.
  • Seiquer I; Departamento Nutrición y Producción Animal Sostenible, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), San Miguel 101, Armilla, 18100 Granada, Spain.
  • Pardo Z; Departamento Nutrición y Producción Animal Sostenible, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), San Miguel 101, Armilla, 18100 Granada, Spain.
  • Haro A; Departamento Nutrición y Producción Animal Sostenible, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), San Miguel 101, Armilla, 18100 Granada, Spain.
  • Recio I; Instituto de Investigación en Ciencias de la Alimentación, CIAL (CSIC-UAM, CEI UAM+CSIC), Nicolás Cabrera, 9, 28049 Madrid, Spain.
  • Olías R; Departamento Nutrición y Producción Animal Sostenible, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), San Miguel 101, Armilla, 18100 Granada, Spain.
Antioxidants (Basel) ; 11(9)2022 Sep 16.
Article em En | MEDLINE | ID: mdl-36139901
Whey-based beverages could be an effective way of reusing a by-product of th cheese industry, mitigating environmental hazards and, at the same time, profiting a useful food with high nutritional and antioxidant properties. In this study, a traditional Ecuadorian beverage (Colada) was prepared combining sweet whey, Maracuyá and barley. Antioxidant properties before and after an in vitro digestion using the INFOGEST method were determined, and relationships with intestinal transformations of the lipid and protein fractions were analyzed. The digestive process had a positive effect on antioxidant properties based on increased values of ABTS and FRAP located in the bioaccessible fraction (BF), together with strong increments of total polyphenols. Moreover, pretreatment of Caco-2 cells with the BF of Colada significantly reduced ROS generation (p < 0.001) measured by the dichlorofluorescein assay. Substantial changes of the fatty acid profile occurred during digestion, such as a fall of saturated fatty acids and a rise of polyunsaturated. The protein profile, examined by SDS-PAGE and exclusion molecular chromatography in the BF, showed that the major part of the proteins were digested in the intestinal phase. Analysis of NanoLC-MS/MS revealed 18 antioxidant peptides originated from whey proteins, but also 16 peptides from barley with potential antioxidant properties. In conclusion, combining sweet whey with Maracuyá and barley constitutes an excellent nutritional beverage with a strong antioxidant potential.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Antioxidants (Basel) Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Equador País de publicação: Suíça

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Antioxidants (Basel) Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Equador País de publicação: Suíça