Your browser doesn't support javascript.
loading
Morphological Changes in Endothelial Cell Organelles in a No-Touch Saphenous Vein Graft.
Sugaya, Akira; Ohno, Nobuhiko; Yashiro, Takashi; Kawahito, Koji.
Afiliação
  • Sugaya A; Department of Cardiovascular Surgery, School of Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan.
  • Ohno N; Department of Anatomy, Division of Histology and Cell Biology, School of Medicine, Jichi Medical University, Shimotsuke, Tochigi, Japan.
  • Yashiro T; Division of Ultrastructural Research, National Institute for Physiological Sciences, Myodaiji-cho, Okazaki, Aichi, Japan.
  • Kawahito K; Nasu School of Nursing, Nasushiobara, Tochigi, Japan.
Braz J Cardiovasc Surg ; 37(Spec 1): 49-56, 2022 09 02.
Article em En | MEDLINE | ID: mdl-36054002
INTRODUCTION: Improved long-term patency of the no-touch (NT) saphenous vein graft has been reported to result from the preservation of a healthy vascular microstructure, especially endothelial cells. However, the precise morphology of endothelial cells and their organelles in NT saphenous vein graft has not been fully investigated. In this study, we assessed the ultrastructure of preserved endothelial cells in saphenous vein graft using transmission electron microscopy. METHODS: Intact control (IC) vein, NT saphenous vein graft, and conventional (CT) saphenous vein graft were harvested from a patient. After observation by light microscopy, the nuclei and mitochondria in the preserved endothelial cells were compared among IC, NT, and CT using transmission electron microscopy, and the endothelial organelles were assessed quantitatively. RESULTS: Light microscopy showed that the preservation of endothelial cells was comparable in IC, NT, and CT. Subsequent transmission electron microscopy observation showed that the nuclei in preserved endothelial cells appeared more swollen in CT than that in NT. Quantitative analysis revealed that nuclear size and circularity of preserved endothelial cells in NT and IC were similar, but those in CT were larger and higher, respectively, than those in IC and NT. In addition, the mitochondrial size in preserved endothelial cells in CT was larger than that in IC and NT. CONCLUSION: Necrotic changes in endothelial organelles characterized by swelling of nuclei and mitochondria were prominent in CT saphenous vein graft. The normally maintained ultrastructure of preserved endothelial cells in NT saphenous vein graft could contribute to long-term patency.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Veia Safena / Ponte de Artéria Coronária Limite: Humans Idioma: En Revista: Braz J Cardiovasc Surg Assunto da revista: ANGIOLOGIA / CARDIOLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Japão País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Veia Safena / Ponte de Artéria Coronária Limite: Humans Idioma: En Revista: Braz J Cardiovasc Surg Assunto da revista: ANGIOLOGIA / CARDIOLOGIA Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Japão País de publicação: Brasil