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TNFAIP3 mediates FGFR1 activation-induced breast cancer angiogenesis by promoting VEGFA expression and secretion.
Gao, Mengdi; Li, Xue; Yang, Mao; Feng, WanRu; Lin, Yan; He, Tao.
Afiliação
  • Gao M; Institute for Cancer Medicine and School of Basic Medical Sciences, Southwest Medical University, Luzhou, 646000, Sichuan, China.
  • Li X; Institute for Cancer Medicine and School of Basic Medical Sciences, Southwest Medical University, Luzhou, 646000, Sichuan, China.
  • Yang M; Institute for Cancer Medicine and School of Basic Medical Sciences, Southwest Medical University, Luzhou, 646000, Sichuan, China.
  • Feng W; Institute for Cancer Medicine and School of Basic Medical Sciences, Southwest Medical University, Luzhou, 646000, Sichuan, China.
  • Lin Y; Institute for Cancer Medicine and School of Basic Medical Sciences, Southwest Medical University, Luzhou, 646000, Sichuan, China. lydemail@swmu.edu.cn.
  • He T; Institute for Cancer Medicine and School of Basic Medical Sciences, Southwest Medical University, Luzhou, 646000, Sichuan, China. hetao198@swmu.edu.cn.
Clin Transl Oncol ; 24(12): 2453-2465, 2022 Dec.
Article em En | MEDLINE | ID: mdl-36002765
PURPOSE: To investigate the role and mechanism of TNF-inducible protein 3(TNFAIP3) in breast cancer angiogenesis induced by fibroblast growth factor receptor1 (FGFR1) activation. METHODS: The immunohistochemical assay was used to detect the expression of vascular endothelial cell marker CD31 and CD105 in mice DCIS.COM-iFGFR1 transplanted tumor (previously established by our group). The effects of TNFAIP3 knockout/knockdown breast cancer cell lines on angiogenesis, migration, and invasion of Human Umbilical Vein Endothelial Cells (HUVEC) were detected by the tubulogenesis and Trewells assay. RNA-seq analysis of TNFAIP3 downstreams differential genes after TNFAIP3 knockdown. The expression and secretion of VEGFA after FGFR1 activation in breast cancer cells were detected by qPCR, Western blot, and ELISA. RESULTS: Immunohistochemistry showed that TNFAIP3 knockout inhibited the expression of CD31 and CD105 in DCIS grafted tumors promoted by FGFR1 activation. Tubulogenesis and Trewells experiments showed that TNFAIP3 gene knockout/knockdown inhibited the angiogenesis, migration, and invasion of HUVEC cells promoted by FGFR1 activation. qPCR assay showed that VEGFA mRNA level in the TNFAIP3 knockdown cell line was significantly down-regulated (p < 0.05). qPCR, Western blot and ELISA results showed that TNFAIP3 gene knockout/knockdown could inhibit the expression and secretion of VEGFA in breast cancer cells induced by FGFR1 activation. CONCLUSION: TNFAIP3 promotes breast cancer angiogenesis induced by FGFR1 activation through the expression and secretion of VEGFA.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Mama / Carcinoma Intraductal não Infiltrante Limite: Animals / Female / Humans Idioma: En Revista: Clin Transl Oncol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China País de publicação: Itália
Texto completo
Adicionar na Minha BVS
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XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Neoplasias da Mama / Carcinoma Intraductal não Infiltrante Limite: Animals / Female / Humans Idioma: En Revista: Clin Transl Oncol Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China País de publicação: Itália