Your browser doesn't support javascript.
loading
CaMKIV/CREB/BDNF signaling pathway expression in prefrontal cortex, amygdala, hippocampus and hypothalamus in streptozotocin-induced diabetic mice with anxious-like behavior.
Espasandín, Camila; Rivero, Sofía; Bengoa, Laura; Cal, Karina; Romanelli, Gerardo; Benech, Juan Claudio; Damián, Juan Pablo.
Afiliação
  • Espasandín C; Departamento de Biociencias Veterinarias, Facultad de Veterinaria, Universidad de la República, Lasplaces 1550, 11600, Montevideo, CP, Uruguay.
  • Rivero S; Laboratorio de Señalización Celular y Nanobiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, 11600, Montevideo, CP, Uruguay.
  • Bengoa L; Departamento de Biociencias Veterinarias, Facultad de Veterinaria, Universidad de la República, Lasplaces 1550, 11600, Montevideo, CP, Uruguay.
  • Cal K; Departamento de Biociencias Veterinarias, Facultad de Veterinaria, Universidad de la República, Lasplaces 1550, 11600, Montevideo, CP, Uruguay.
  • Romanelli G; Departamento de Biociencias Veterinarias, Facultad de Veterinaria, Universidad de la República, Lasplaces 1550, 11600, Montevideo, CP, Uruguay.
  • Benech JC; Laboratorio de Patologías del Metabolismo y el Envejecimiento, Institut Pasteur Montevideo, Mataojo 2020, 11400, Montevideo, CP, Uruguay.
  • Damián JP; Laboratorio de Señalización Celular y Nanobiología, Instituto de Investigaciones Biológicas Clemente Estable, Avenida Italia 3318, 11600, Montevideo, CP, Uruguay.
Exp Brain Res ; 240(10): 2687-2699, 2022 Oct.
Article em En | MEDLINE | ID: mdl-35984483
Individuals with diabetes mellitus (DM) tend to manifest anxiety and depression, which could be related to changes in the expression of calcium/calmodulin-dependent protein kinase IV (CaMKIV), transcription factor cyclic AMP-responsive element binding protein (CREB), phosphorylated CREB (pCREB) and brain-derived neurotrophic factor (BDNF) in different brain regions. The objective of this study was to determine whether mice with type 1 diabetes (T1DM) induced with streptozotocin show a profile of anxious-type behaviors and alterations in the expression/activity of CaMKIV, CREB, pCREB and BDNF in different regions of the brain (prefrontal cortex, amygdala, hippocampus and hypothalamus) in comparison to non-diabetic mice (NDB). Mice with 3 months of chronic DM showed an anxious-like behavioral profile in two anxiety tests (Open Field and Elevated Plus Maze), when compared to NDB. There were significant differences in the expression of cell signaling proteins: diabetic mice had a lower expression of CaMKIV in the hippocampus, a greater expression of CREB in the amygdala and hypothalamus, as well as a lower pCREB/CREB in hypothalamus than NDB mice (P < 0.05). This is the first study evaluating the expression of CaMKIV in the brain of animals with DM, who presented lower expression of this protein in the hippocampus. In addition, it is the first time that CREB was evaluated in amygdala and hypothalamus of animals with DM, who presented a higher expression. Further research is necessary to determine the possible link between expression of CaMKIV and CREB, and the behavioral profile of anxiety in diabetic animals.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fator Neurotrófico Derivado do Encéfalo / Diabetes Mellitus Limite: Animals Idioma: En Revista: Exp Brain Res Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Uruguai País de publicação: Alemanha
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Fator Neurotrófico Derivado do Encéfalo / Diabetes Mellitus Limite: Animals Idioma: En Revista: Exp Brain Res Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Uruguai País de publicação: Alemanha