Your browser doesn't support javascript.
loading
Analysis of Crassostrea gasar transcriptome reveals candidate genes involved in metal metabolism.
Ferreira, Clarissa P; Moreira, Renato S; Toledo-Silva, Guilherme; Schroeder, Declan C; Bainy, Afonso C D; Lüchmann, Karim H.
Afiliação
  • Ferreira CP; Multicentric Graduate Program in Biochemistry and Molecular Biology - PMBqBM, Santa Catarina State University, Lages, 88520-000, Brazil.
  • Moreira RS; Federal Institute of Santa Catarina - IFSC, Lages, 88506-400, Brazil.
  • Toledo-Silva G; Genomics Laboratory, Cell Biology, Embryology and Genetics Department, Federal University of Santa Catarina, Florianópolis, 88040-900, Brazil.
  • Schroeder DC; Department of Veterinary Population Medicine, University of Minnesota, St. Paul, MN, 55108, USA; School of Biological Sciences, University of Reading, Reading, UK.
  • Bainy ACD; Laboratory of Biomarkers of Aquatic Contamination and Immunochemistry - LABCAI, Federal University of Santa Catarina, Florianópolis, 88034-257, Brazil.
  • Lüchmann KH; Department of Scientific and Technological Education, Santa Catarina State University, Florianópolis, 88035-001, Brazil. Electronic address: karim.luchmann@udesc.br.
Chemosphere ; 307(Pt 4): 136009, 2022 Nov.
Article em En | MEDLINE | ID: mdl-35977572
Oysters have been extensively employed for monitoring of metal pollution in dynamic aquatic ecosystems. Therefore, the use of specific biomarkers can assist in discriminating the ecotoxicological implications of different elements in such complex environments. In this study, we revisited the sequencing data of gills and digestive glands transcripts in the mangrove oyster Crassostrea gasar and generated a reference transcriptome assembly from multiple assemblers, seven in total. Overall, we were able to identify a total of 11,917 transcripts, with 86.6% of them being functionally annotated and 1.4 times more than the first annotation. We screened the annotated transcripts to identify genes potentially involved in metals' transport, storage, and detoxification. Our findings included genes related to Zn distribution in cells (Zn transporters - ZIP, ZnT), metallothionein (MT-I and MT-IV), GSH biosynthesis, Ca+ transporter (NCX and ATP2B), and Cu distribution in cells (ATP7, ATOX1, CCS, and laccase-like). These results provided a reference transcriptome for additional insights into the transcriptional profile of C. gasar and other bivalves to better understand the molecular pathways underpinning metal tolerance and susceptibility. The study also provided an auxiliary tool for biomonitoring metal contamination in dynamic environments as estuaries.
Assuntos
Palavras-chave
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poluentes Químicos da Água / Crassostrea Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Chemosphere Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Poluentes Químicos da Água / Crassostrea Tipo de estudo: Prognostic_studies Limite: Animals Idioma: En Revista: Chemosphere Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido