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Foxa1 mediates eccrine sweat gland development through transcriptional regulation of Na-K-ATPase expression.
Zhao, Junhong; Zhang, Lei; Du, Lijie; Chen, Zixiu; Tang, Yue; Chen, Lijun; Liu, Xiang; You, Lei; Zhang, Yonghong; Fu, Xiaobing; Li, Haihong.
Afiliação
  • Zhao J; Department of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
  • Zhang L; Mental Health Center, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
  • Du L; Department of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
  • Chen Z; Department of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
  • Tang Y; Department of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
  • Chen L; Department of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
  • Liu X; Department of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
  • You L; School of Basic Medicine, Academy of Bio-Medicine Research, Hubei University of Medicine, Shiyan, Hubei, China.
  • Zhang Y; School of Basic Medicine, Academy of Bio-Medicine Research, Hubei University of Medicine, Shiyan, Hubei, China.
  • Fu X; Wound Healing and Cell Biology Laboratory, The First Affiliated Hospital, Chinese PLA General Hospital, Beijing, China.
  • Li H; Department of Wound Repair and Dermatologic Surgery, Taihe Hospital, Hubei University of Medicine, Shiyan, Hubei, China.
Braz J Med Biol Res ; 55: e12149, 2022.
Article em En | MEDLINE | ID: mdl-35976271
Eccrine sweat glands (ESGs) perform critical functions in temperature regulation in humans. Foxa1 plays an important role in ESG maturation and sweat secretion. Its molecular mechanism, however, remains unknown. This study investigated the expression of Foxa1 and Na-K-ATPase (NKA) in rat footpads at different development stages using immunofluorescence staining, qRT-PCR, and immunoblotting. Also, bioinformatics analysis and Foxa1 overexpression and silencing were employed to evaluate Foxa1 regulation of NKA. The results demonstrated that Foxa1 was consistently expressed during the late stages of ESGs and had a significant role in secretory coil maturation during sweat secretion. Furthermore, the mRNA abundance and protein expression of NKA had similar accumulation trends to those of Foxa1, confirming their underlying connections. Bioinformatics analysis revealed that Foxa1 may interact with these two proteins via binding to conserved motifs in their promoter regions. Foxa1 gain-of-function and loss-of-function experiments in Foxa1-modified cells demonstrated that the activities of NKA were dependent on the presence of Foxa1. Collectively, these data provided evidence that Foxa1 may influence ESG development through transcriptional regulation of NKA expression.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regulação da Expressão Gênica / Glândulas Écrinas Limite: Animals / Humans Idioma: En Revista: Braz J Med Biol Res Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China País de publicação: Brasil
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Regulação da Expressão Gênica / Glândulas Écrinas Limite: Animals / Humans Idioma: En Revista: Braz J Med Biol Res Ano de publicação: 2022 Tipo de documento: Article País de afiliação: China País de publicação: Brasil