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Changes in fatty acid composition as a response to glyphosate toxicity in Pseudomonas fluorescens.
de Oliveira, Elizangela Paz; Marchi, Kathleen Evelyn; Emiliano, Janaina; Salazar, Stella Marys Christóforo Hinojosa; Ferri, Alisson Henrique; Etto, Rafael Mazer; Reche, Péricles Martim; Pileggi, Sônia Alvim Veiga; Kalks, Karlos Henrique Martins; Tótola, Marcos Rogério; Schemczssen-Graeff, Zelinda; Pileggi, Marcos.
Afiliação
  • de Oliveira EP; Department of Biotechnology, Genetics and Cell Biology, Maringá State University, Maringá, Paraná, Brazil.
  • Marchi KE; Department of Structural and Molecular Biology and Genetics, Ponta Grossa State University, Ponta Grossa, Paraná, Brazil.
  • Emiliano J; Department of Microbiology, Londrina State University, Londrina, Paraná, Brazil.
  • Salazar SMCH; Department of Structural and Molecular Biology and Genetics, Ponta Grossa State University, Ponta Grossa, Paraná, Brazil.
  • Ferri AH; Department of Structural and Molecular Biology and Genetics, Ponta Grossa State University, Ponta Grossa, Paraná, Brazil.
  • Etto RM; Department of Chemistry, Ponta Grossa State University, Ponta Grossa, Paraná, Brazil.
  • Reche PM; Department of Nursing and Public Health, Ponta Grossa State University, Ponta Grossa, Paraná, Brazil.
  • Pileggi SAV; Department of Structural and Molecular Biology and Genetics, Ponta Grossa State University, Ponta Grossa, Paraná, Brazil.
  • Kalks KHM; Department of Microbiology, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil.
  • Tótola MR; Department of Microbiology, Federal University of Viçosa, Viçosa, Minas Gerais, Brazil.
  • Schemczssen-Graeff Z; Department of Cellular Biology, Federal University of Paraná, Curitiba, Paraná, Brazil.
  • Pileggi M; Department of Structural and Molecular Biology and Genetics, Ponta Grossa State University, Ponta Grossa, Paraná, Brazil.
Heliyon ; 8(8): e09938, 2022 Aug.
Article em En | MEDLINE | ID: mdl-35965982
Excessive use of herbicides decreases soil biodiversity and fertility. The literature on the xenobiotic response by microorganisms is focused on herbicide biodegradation as a selective event. Non-degradation systems independent of selection could allow the survival of tolerant bacteria in contaminated environments, impacting xenobiotic turnover and, consequently, bioremediation strategies. However, it is uncertain whether the response based on these systems requires selective pressure to be effective. The objective here was to analyze non-degradation phenotypes, enzymatic and structural response systems, of Pseudomonas fluorescens CMA-55 strain, already investigated the production pattern of quorum sensing molecules in response to glyphosate, not present at the isolation site. One mode of response was associated with decrease in membrane permeability and effective antioxidative response for 0-2.30 mM glyphosate, at the mid-log growing phase, with higher activities of Mn-SOD, KatA, and KatB, and presence of fatty acids as nonadecylic acid, margaric and lauric acid. The second response system was characterized by lower antioxidative enzymes activity, presence of KatC isoform, and pelargonic, capric, myristic, stearic, palmitoleic and palmitic acid as principal fatty acids, allowing the strain to face stressful conditions in 9.20-11.50 mM glyphosate at the stationary phase. Therefore, the bacterial strain could modify the fatty acid composition and the permeability of membranes in two response modes according to the herbicide concentration, even glyphosate was not previously selective for P. fluorescens, featuring a generalist system based on physiological plasticity.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Heliyon Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido
Texto completo
Adicionar na Minha BVS
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XML
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Idioma: En Revista: Heliyon Ano de publicação: 2022 Tipo de documento: Article País de afiliação: Brasil País de publicação: Reino Unido