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Anther development in Brachiaria brizantha (syn. Urochloa brizantha) and perspective for microspore in vitro culture.
Koehler, Andréa D; Rossi, Mônica L; Carneiro, Vera T C; Cabral, Glaucia B; Martinelli, Adriana P; Dusi, Diva M A.
Afiliação
  • Koehler AD; University of Sao Paulo, CENA, Av. Centenario 303, Piracicaba, SP, 13416-903, Brazil.
  • Rossi ML; Brazilian Agricultural Research Corporation (Embrapa), Embrapa Genetic Resources and Biotechnology, Cx.Postal 02372, Brasilia, DF, 70.770-917, Brazil.
  • Carneiro VTC; University of Sao Paulo, CENA, Av. Centenario 303, Piracicaba, SP, 13416-903, Brazil.
  • Cabral GB; Brazilian Agricultural Research Corporation (Embrapa), Embrapa Genetic Resources and Biotechnology, Cx.Postal 02372, Brasilia, DF, 70.770-917, Brazil.
  • Martinelli AP; Brazilian Agricultural Research Corporation (Embrapa), Embrapa Genetic Resources and Biotechnology, Cx.Postal 02372, Brasilia, DF, 70.770-917, Brazil.
  • Dusi DMA; University of Sao Paulo, CENA, Av. Centenario 303, Piracicaba, SP, 13416-903, Brazil.
Protoplasma ; 260(2): 571-587, 2023 Mar.
Article em En | MEDLINE | ID: mdl-35947212
Brachiaria, a genus from the Poaceae family, is largely cultivated as forage in Brazil. Among the most cultivated varieties of Brachiaria spp., B. brizantha cv. Marandu (syn. Urochloa brizantha) is of great agronomical importance due to the large areas cultivated with this species. This cultivar is apomictic and tetraploid. Sexual diploid genotype is available for this species. The difference in levels of ploidy among sexual and apomictic plants contributes to hindering Brachiaria breeding programs. The induction of haploids and double haploids is of great interest for the generation of new genotypes with potential use in intraspecific crosses. A key factor for the success of this technique is identifying adequate microspore developmental stages for efficient embryogenesis induction. Knowledge of the morphological changes during microsporogenesis and microgametogenesis and sporophytic tissues composing the anther is critical for identifying the stages in which microspores present a higher potential for embryogenic callus and somatic embryo through in vitro culture. In this work, morphological markers were associated with anther and pollen grain developmental stages, through histological analysis. Anther development was divided into 11 stages using morphological and cytological characteristics, from anther with archesporial cells to anther dehiscence. The morphological characteristics of each stage are presented. In addition, the response of stage 8 anthers to in vitro culture indicates microspores initiating somatic embryogenic pathway.
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Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Brachiaria Tipo de estudo: Prognostic_studies Idioma: En Revista: Protoplasma Assunto da revista: BIOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Áustria
Texto completo
Adicionar na Minha BVS
Imprimir
XML
PubMed Links
Buscar no Google

Texto completo: 1 Coleções: 01-internacional Base de dados: MEDLINE Assunto principal: Brachiaria Tipo de estudo: Prognostic_studies Idioma: En Revista: Protoplasma Assunto da revista: BIOLOGIA Ano de publicação: 2023 Tipo de documento: Article País de afiliação: Brasil País de publicação: Áustria